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<p>The N-terminal tandem RNA Recognition Motifs (RRM1−2) of the splicing factor RNA Binding Motif 39 (RBM39) from <i>Homo sapiens</i> was expressed in <i>E. coli</i> with <sup>13</sup>C and <sup>15</sup>N labeling. A nearly complete assignment of backbone <sup>1</sup>H, <sup>15</sup>N, and <sup>13</sup>C resonances was obtained, along with a secondary structure prediction based on the assigned chemical shifts. The predicted secondary structures of the RBM39 tandem RRM1−2 closely matched the published solution structures of the individual RBM39 RRM1 and RRM2, and also aligned well with the AlphaFold2 model of RBM39.</p>

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1H, 13C and 15N backbone resonance assignment of the RNA binding motif 39 (RBM39) tandem RNA recognition motifs (RRM1−2)

  • 期刊论文

  • 发表日期：

    2025年9月13日

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  • Florian Malard,
  • Léa Bouton,
  • Sébastien Campagne

摘要

The N-terminal tandem RNA Recognition Motifs (RRM1−2) of the splicing factor RNA Binding Motif 39 (RBM39) from Homo sapiens was expressed in E. coli with 13C and 15N labeling. A nearly complete assignment of backbone 1H, 15N, and 13C resonances was obtained, along with a secondary structure prediction based on the assigned chemical shifts. The predicted secondary structures of the RBM39 tandem RRM1−2 closely matched the published solution structures of the individual RBM39 RRM1 and RRM2, and also aligned well with the AlphaFold2 model of RBM39.