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<p>Due to the emergence of the SARS-CoV-2 virus, research on coronaviruses has been massively accelerated. In addition to SARS-CoV-2, there are other human coronaviruses, including HCoV-229E. In all coronaviruses, secondary structure predictions indicate the presence of conserved structural elements in the 5’-untranslated region (5’-UTR). These conserved elements play crucial roles in RNA translation and replication. Stem-loop 5 (SL5), consisting of three substructures (5a, 5b, 5c), is highly conserved and harbours the start codon for translation. SL5 has repetitive structural motifs (RSMs), 5’-UUYYGU-3’, which are conserved in many alpha- and betacoronaviruses. In the following, we present the <sup>1</sup>H, <sup>13</sup>C and <sup>15</sup>N NMR resonance assignment of the SL5a RNA element from HCoV-229E and variations in the RSMs to show the effect of loop mutations on the structure of the hexaloop, revealing the different impact of each loop nucleotide on RNA dynamics.</p>

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1H, 13C and 15N chemical shift assignment for stem-loop 5a from the 5‘UTR of HCoV-229E

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月31日

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  • Nina M. Krause,
  • Anna Wacker,
  • Christian Richter,
  • Boris Fürtig,
  • Ramakanth Madhugiri,
  • John Ziebuhr,
  • Harald Schwalbe

摘要

Due to the emergence of the SARS-CoV-2 virus, research on coronaviruses has been massively accelerated. In addition to SARS-CoV-2, there are other human coronaviruses, including HCoV-229E. In all coronaviruses, secondary structure predictions indicate the presence of conserved structural elements in the 5’-untranslated region (5’-UTR). These conserved elements play crucial roles in RNA translation and replication. Stem-loop 5 (SL5), consisting of three substructures (5a, 5b, 5c), is highly conserved and harbours the start codon for translation. SL5 has repetitive structural motifs (RSMs), 5’-UUYYGU-3’, which are conserved in many alpha- and betacoronaviruses. In the following, we present the 1H, 13C and 15N NMR resonance assignment of the SL5a RNA element from HCoV-229E and variations in the RSMs to show the effect of loop mutations on the structure of the hexaloop, revealing the different impact of each loop nucleotide on RNA dynamics.