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<p>Flap endonuclease (FEN) enzymes are a group of metallonucleases that have essential roles in DNA repair and the maintenance of genomic resilience. They catalyse hydrolytic cleavage of a phosphodiester bond to remove 5′-flaps present on double-stranded DNA molecules formed during DNA replication. FEN locates a target scissile bond through the structural recognition of a pronounced bend in the double-stranded DNA substrate along with the presence of both a 5′-flap and a 1-nucleotide 3′-flap. FEN enzymes share a common structural architecture and are functionally conserved across all living organisms. In this work, we report the <sup>1</sup>H, <sup>15</sup>N and <sup>13</sup>C backbone resonance assignments of residues 2–349 of FEN from <i>Plasmodium falciparum</i> (<i>Pf</i>FEN349) in its substrate-free state. Using heteronuclear multidimensional NMR spectroscopy, 90% of all backbone resonances of <i>Pf</i>FEN349 were assigned, with 298 backbone amide resonances out of 337 theoretically-detectible resonances (which exclude 10 prolines and the N-terminal glycine) identified in the <sup>1</sup>H–<sup>15</sup>&#xa0;N TROSY spectrum. Prediction of solution secondary structure content from a chemical shift analysis using the TALOS-N webserver is mostly in good agreement with an AlphaFold model of <i>Pf</i>FEN349. The reported assignments provide a pathway for drug discovery as <i>Pf</i>FEN349 is a potential target for the development of new inhibitors that could be utilised to control the incidence of malaria across the globe.</p>

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1H, 15N and 13C backbone resonance assignments of flap endonuclease from Plasmodium falciparum

  • 期刊论文

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  • 发表日期：

    2025年8月9日

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  • Rodolpho do Aido-Machado,
  • Nicola J. Baxter,
  • Michelle L. Rowe,
  • Manoj B. Pohare,
  • Srdjan Vitovski,
  • Jon R. Sayers,
  • Jonathan P. Waltho

摘要

Flap endonuclease (FEN) enzymes are a group of metallonucleases that have essential roles in DNA repair and the maintenance of genomic resilience. They catalyse hydrolytic cleavage of a phosphodiester bond to remove 5′-flaps present on double-stranded DNA molecules formed during DNA replication. FEN locates a target scissile bond through the structural recognition of a pronounced bend in the double-stranded DNA substrate along with the presence of both a 5′-flap and a 1-nucleotide 3′-flap. FEN enzymes share a common structural architecture and are functionally conserved across all living organisms. In this work, we report the 1H, 15N and 13C backbone resonance assignments of residues 2–349 of FEN from Plasmodium falciparum (PfFEN349) in its substrate-free state. Using heteronuclear multidimensional NMR spectroscopy, 90% of all backbone resonances of PfFEN349 were assigned, with 298 backbone amide resonances out of 337 theoretically-detectible resonances (which exclude 10 prolines and the N-terminal glycine) identified in the 1H–15 N TROSY spectrum. Prediction of solution secondary structure content from a chemical shift analysis using the TALOS-N webserver is mostly in good agreement with an AlphaFold model of PfFEN349. The reported assignments provide a pathway for drug discovery as PfFEN349 is a potential target for the development of new inhibitors that could be utilised to control the incidence of malaria across the globe.