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Objectives <p>Rotavirus is the most frequent cause of severe acute gastroenteritis (AGE) in children aged under 5 y in India. This study aimed to determine the proportion of rotavirus gastroenteritis in children aged under 5 y admitted with AGE and to characterize the circulating genotypes after implementation of rotavirus vaccination within the Universal Immunization Program (UIP) in Karnataka, India.</p> Methods <p>This multicenter, cross-sectional observational study included all children aged under 5 y with AGE admitted to four hospitals in Karnataka. Their demographic and clinical characteristics were recorded using a predesigned electronic form. Stool samples were tested for rotavirus using an enzyme-linked immunosorbent assay (ELISA), and all ELISA-positive samples were genotyped using polymerase chain reaction.</p> Results <p>Of the 433 children enrolled, 405 had a stool sample tested for rotavirus, of which 97 (23.9%) were positive. The prevalence of rotavirus was highest among children aged 12–23 mo (41.2%), followed by infants aged 24–59 mo (26.8%). Among the 97 rotavirus-positive children, the predominant clinical manifestations were vomiting (95.9%), fever (79.4%), and severe to very severe dehydration (87.6%). G3P[8] (54.2%) was the most common genotype, followed by G1P[6] (17.8%) and G2P[4] (11.5%).</p> Conclusions <p>Of the children admitted with AGE, 23.9% tested positive for rotavirus, with the highest burden in children aged 12–23 mo. The predominant genotypes were G3P[8], G1P[6], and G2P[4]. Ongoing genotypic surveillance is warranted to assess the impact of rotavirus vaccination on the incidence of rotavirus AGE and circulating rotavirus genotypes in the population to inform rotavirus vaccination strategy.</p>

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Clinico-Epidemiological Profile and Genetic Characterization of Rotavirus Strains Among Children Aged Under 5 Years Hospitalized with Acute Gastroenteritis in Karnataka, India

  • 期刊论文

  • 发表日期：

    2025年9月9日

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  • Hassan Sreenivasa Murthy Rajani,
  • Namburu Kiran,
  • Harika Marri,
  • Wari Prakash,
  • Illalu Shivanand,
  • Cholachagudda Shilpa,
  • Nutan Kamath,
  • Suchetha Rao,
  • Varsha Sudhir Chaudhary,
  • Anupama Machathi,
  • Namrata Kharat,
  • Poovarasan Kannan,
  • Niranjana Mahantashetti,
  • Tintu Varghese

摘要

Objectives

Rotavirus is the most frequent cause of severe acute gastroenteritis (AGE) in children aged under 5 y in India. This study aimed to determine the proportion of rotavirus gastroenteritis in children aged under 5 y admitted with AGE and to characterize the circulating genotypes after implementation of rotavirus vaccination within the Universal Immunization Program (UIP) in Karnataka, India.

Methods

This multicenter, cross-sectional observational study included all children aged under 5 y with AGE admitted to four hospitals in Karnataka. Their demographic and clinical characteristics were recorded using a predesigned electronic form. Stool samples were tested for rotavirus using an enzyme-linked immunosorbent assay (ELISA), and all ELISA-positive samples were genotyped using polymerase chain reaction.

Results

Of the 433 children enrolled, 405 had a stool sample tested for rotavirus, of which 97 (23.9%) were positive. The prevalence of rotavirus was highest among children aged 12–23 mo (41.2%), followed by infants aged 24–59 mo (26.8%). Among the 97 rotavirus-positive children, the predominant clinical manifestations were vomiting (95.9%), fever (79.4%), and severe to very severe dehydration (87.6%). G3P[8] (54.2%) was the most common genotype, followed by G1P[6] (17.8%) and G2P[4] (11.5%).

Conclusions

Of the children admitted with AGE, 23.9% tested positive for rotavirus, with the highest burden in children aged 12–23 mo. The predominant genotypes were G3P[8], G1P[6], and G2P[4]. Ongoing genotypic surveillance is warranted to assess the impact of rotavirus vaccination on the incidence of rotavirus AGE and circulating rotavirus genotypes in the population to inform rotavirus vaccination strategy.