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Purpose <p>This study aimed to investigate the expression of infl ammatory bowel disease (IBD)-associated genes in oral cancer and to elucidate the cellular and molecular pathways that may serve as potential therapeutic targets.</p> Methods/Patients <p>Oral cancer tissue samples were subjected to single-cell RNA sequencing to characterize cell clusters and gene expression patterns. UMAP and t-SNE were used for dimensionality reduction and visualization of cellular subgroups. Dot plots were applied to identify key gene expression signatures. Gene Set Enrichment Analysis (GSEA) was performed to examine pathways associated with NFKBIA expression.</p> Results <p>Single-cell analysis revealed heterogeneous cell populations, including T cells, fi broblasts, and malignant cells. Diff erential expression analysis identifi ed elevated levels of LYZ and IL7R in specifi c cell subsets. In several clusters, IBD-related genes such as NFKBIA, RB1CC1, and ATG5 were upregulated. GSEA indicated that NFKBIA expression was signifi cantly associated with the chemokine-mediatedsignaling pathway, a process implicated in tumor growth.</p> Conclusions <p>This study highlights the cellular heterogeneity and distinct gene expression programs inoral cancer, emphasizing the relevance of IBD-associated genes. The association between NFKBIA expression and chemokine-mediated signaling provides insights that may contribute to the development of personalized therapeutic strategies for IBD-related mechanisms in oral cancer.</p>

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Investigating the role of inflammatory bowel disease-associated gene expression in oral cancer using single-cell RNA sequencing

  • 期刊论文

  • 发表日期：

    2025年12月15日

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  • Yongwei Cheng,
  • Zhenyin Liu,
  • Shifeng Xie,
  • Ningyi Zhang,
  • Liang Guo,
  • Yujin Wu,
  • Yuan Liao,
  • Yunkai Dai

摘要

Purpose

This study aimed to investigate the expression of infl ammatory bowel disease (IBD)-associated genes in oral cancer and to elucidate the cellular and molecular pathways that may serve as potential therapeutic targets.

Methods/Patients

Oral cancer tissue samples were subjected to single-cell RNA sequencing to characterize cell clusters and gene expression patterns. UMAP and t-SNE were used for dimensionality reduction and visualization of cellular subgroups. Dot plots were applied to identify key gene expression signatures. Gene Set Enrichment Analysis (GSEA) was performed to examine pathways associated with NFKBIA expression.

Results

Single-cell analysis revealed heterogeneous cell populations, including T cells, fi broblasts, and malignant cells. Diff erential expression analysis identifi ed elevated levels of LYZ and IL7R in specifi c cell subsets. In several clusters, IBD-related genes such as NFKBIA, RB1CC1, and ATG5 were upregulated. GSEA indicated that NFKBIA expression was signifi cantly associated with the chemokine-mediatedsignaling pathway, a process implicated in tumor growth.

Conclusions

This study highlights the cellular heterogeneity and distinct gene expression programs inoral cancer, emphasizing the relevance of IBD-associated genes. The association between NFKBIA expression and chemokine-mediated signaling provides insights that may contribute to the development of personalized therapeutic strategies for IBD-related mechanisms in oral cancer.