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<p>The Chatkal State Biosphere Reserve in the Western Tien Shan is famous for its remarkable plant diversity, yet the endophytic bacteria living within its medicinal plants remain largely unexplored. In this study, we focused on isolating and characterizing plant growth-promoting endophytic bacteria (PGPR) from two widely distributed medicinal species, <i>Glycyrrhiza glabra</i> L. and <i>Hypericum perforatum</i> L. Using 16S rRNA sequencing, we identified several bacterial strains, predominantly from the genera <i>Bacillus</i> and <i>Pseudomonas</i>. These strains displayed multiple traits beneficial to plants, including ammonia production, phosphate solubilization (ranging from 4.3 to 18.1&#xa0;mm), and synthesis of indole-3-acetic acid (IAA). Notably, IAA production increased with tryptophan supplementation (2–8&#xa0;µg/mL), reaching up to 62.5 ± 1.2&#xa0;µg/mL in B. thuringiensis, 58.1 ± 1.3&#xa0;µg/mL in <i>B. amyloliquefaciens</i> and 57.8 ± 2.3&#xa0;µg/mL in B. velezensis. When wheat (<i>Triticum aestivum</i> L.) seeds were inoculated, root lengths increased dramatically, with <i>B. amyloliquefaciens B. atrophaeus</i> and <i>B. licheniformis</i> producing roots of 4.7–5.0&#xa0;cm compared to 0.5–0.8&#xa0;cm in controls. The combination of strains (consortium) resulted in the longest roots (~ 6.0&#xa0;cm), suggesting synergistic interactions. Greenhouse experiments confirmed enhanced shoot and root biomass, with shoot fresh weight increasing by up to 33% and root fresh weight by up to 22%. These findings highlight the functional diversity of endophytic bacteria in Chatkal medicinal plants and their promising potential as biofertilizers for sustainable agriculture.</p> Graphical Abstract <p></p>

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Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Medicinal Plants (Glycyrrhiza glabra L. and Hypericum perforatum L.)

  • 期刊论文

  • 发表日期：

    2026年1月17日

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  • Bahodir Mamarasulov,
  • Kakhramon Davranov,
  • Baljeet Singh Saharan,
  • Minakshi Prasad,
  • Amjad M. Husaini,
  • Dilfuza Jabborova

摘要

The Chatkal State Biosphere Reserve in the Western Tien Shan is famous for its remarkable plant diversity, yet the endophytic bacteria living within its medicinal plants remain largely unexplored. In this study, we focused on isolating and characterizing plant growth-promoting endophytic bacteria (PGPR) from two widely distributed medicinal species, Glycyrrhiza glabra L. and Hypericum perforatum L. Using 16S rRNA sequencing, we identified several bacterial strains, predominantly from the genera Bacillus and Pseudomonas. These strains displayed multiple traits beneficial to plants, including ammonia production, phosphate solubilization (ranging from 4.3 to 18.1 mm), and synthesis of indole-3-acetic acid (IAA). Notably, IAA production increased with tryptophan supplementation (2–8 µg/mL), reaching up to 62.5 ± 1.2 µg/mL in B. thuringiensis, 58.1 ± 1.3 µg/mL in B. amyloliquefaciens and 57.8 ± 2.3 µg/mL in B. velezensis. When wheat (Triticum aestivum L.) seeds were inoculated, root lengths increased dramatically, with B. amyloliquefaciens B. atrophaeus and B. licheniformis producing roots of 4.7–5.0 cm compared to 0.5–0.8 cm in controls. The combination of strains (consortium) resulted in the longest roots (~ 6.0 cm), suggesting synergistic interactions. Greenhouse experiments confirmed enhanced shoot and root biomass, with shoot fresh weight increasing by up to 33% and root fresh weight by up to 22%. These findings highlight the functional diversity of endophytic bacteria in Chatkal medicinal plants and their promising potential as biofertilizers for sustainable agriculture.

Graphical Abstract