<p>The prevalence of cariogenic bacteria in dental caries is attributed to acidification of the oral microenvironment. Cariogenic <i>Pseudomonas aeruginosa</i> strains were isolated from dental caries. A total of 101 <i>P. aeruginosa</i> strains were isolated from 55 samples (<i>n</i> = 55). The isolates were classified based on their acid tolerance and acidogenic properties. The isolated bacterial strains were cultured in soy peptone (2%) and maintained in a simulated oral environment. The survival rate of the isolates varied from 40.2 ± 2.1 to 74.7 ± 1.8%, and only 11 strains had a significant survival rate (&gt; 60%). Among these DS47 strain exhibited strong biofilm production, with an optical density value significantly higher than that of the other isolates (<i>p</i> &lt; 0.01). This strain was further tested to determine its demineralization effect on teeth. The biofilm-forming bacteria DS47 released Ca²⁺ ions in a time-dependent manner (<i>p</i> &lt; 0.01), indicating its potential role in enamel demineralization. Additionally, the DS47 isolate was found to be multidrug-resistant, showing resistance to tobramycin, cefotaxime, ceftazidime, amoxicillin, trimethoprim, ciprofloxacin, levofloxacin, amikacin, ceftazidime, cefoxitin, and gentamicin. The New Delhi metallo β-lactamase-1 (NDM-1) gene and blaVIM-1 genes were detected in the drug-resistant <i>P. aeruginosa</i> DS47. The biofilm-producing <i>P. aeruginosa</i> showed cariogenicity and contributed to dental caries. These findings suggest that biofilm-producing <i>P. aeruginosa</i> plays a significant role in cariogenicity and contributes to dental caries.</p>