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<p>Over the past few years, sepsis, characterized by an exaggerated response to infection, has emerged as a significant global cause of mortality. The rise in antibiotic-resistant strains underscores the necessity to explore alternative approaches beyond antibiotics, which is the conventional treatment. Recent investigations into the human gut microbiome propose considering it as a potential therapeutic alternative.&#xa0;The study was performed on 10 blood samples infected with <i>Staphylococcus aureus</i> ATCC 25923 by treating it with different concentrations of partially purified protein isolated from <i>Lactobacillus</i> spp. Proteins were isolated and purified using a lysis buffer followed by&#xa0;35–40% ammonium sulphate precipitation and dialysis. The protein efficacy was measured via cytotoxicity and cell viability assays such as MTT&#xa0;and dye exclusion assay. The 1:8 and 1:10 <i>S. aureus</i>-to-protein ratio was found to be highly effective in controlling and inhibiting the growth of <i>S. aureus</i>. A statistical analysis by ANOVA determined the effect of age and gender on the blood cells infected with&#xa0;1:8 and 1:10 <i>S. aureus</i>-to-protein samples. The results represent the concentration of protein added to the transfected sample reduced the damage by protecting the systemic immune cells. This overview specifically examines potential proteins within the human gut microbiome that may regulate <i>S. aureus</i>-induced sepsis.&#xa0;This investigative study is an advantage as gut microbiome used can be a novel therapeutic focus to mitigate over activation of inflammasomes during sepsis.</p>

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Lactobacillus spp. from the Human Gut Microbiota as a Therapeutic Tool in Controlling MRSA-Induced Sepsis

  • 期刊论文

  • 发表日期：

    2025年6月30日

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  • Aditi Giriraj,
  • Apoorva Bhat,
  • Sasmita Sabat

摘要

Over the past few years, sepsis, characterized by an exaggerated response to infection, has emerged as a significant global cause of mortality. The rise in antibiotic-resistant strains underscores the necessity to explore alternative approaches beyond antibiotics, which is the conventional treatment. Recent investigations into the human gut microbiome propose considering it as a potential therapeutic alternative. The study was performed on 10 blood samples infected with Staphylococcus aureus ATCC 25923 by treating it with different concentrations of partially purified protein isolated from Lactobacillus spp. Proteins were isolated and purified using a lysis buffer followed by 35–40% ammonium sulphate precipitation and dialysis. The protein efficacy was measured via cytotoxicity and cell viability assays such as MTT and dye exclusion assay. The 1:8 and 1:10 S. aureus-to-protein ratio was found to be highly effective in controlling and inhibiting the growth of S. aureus. A statistical analysis by ANOVA determined the effect of age and gender on the blood cells infected with 1:8 and 1:10 S. aureus-to-protein samples. The results represent the concentration of protein added to the transfected sample reduced the damage by protecting the systemic immune cells. This overview specifically examines potential proteins within the human gut microbiome that may regulate S. aureus-induced sepsis. This investigative study is an advantage as gut microbiome used can be a novel therapeutic focus to mitigate over activation of inflammasomes during sepsis.