Optimization of the Production Parameters of a Novel Exopolysaccharide Chrysosporine from Endophytic Chrysosporium sp. KTL2, and Evaluation of its Antioxidative and Prebiotic Potentialities

Chrysosporine, an exopolysaccharide, was produced by Chrysosporium articulatum KTL2, an endophyte of Ocimum sanctum L. It comprises arabinose, glucose, galactose, and fucose in a molar ratio of approximately 1:5:2:2 with a Mw ~ 2.95 × 10⁵ Da. One Variable at A Time, and Response Surface Quadratic Methodology analysed the maximum production of Chrysosporine at optimised laboratory conditions- 14 days of fermentation at 28 °C in fresh potato dextrose broth in a 250 mL conical flask with 50 mL medium provided with (g L⁻¹) glucose, 12; tryptone concentration, 1.75; NaCl, 0.05; with medium pH 6.5. Further addition of epigenetic modulator- Histone Methyltransferase Specific Probe BRD4770 promotes a 1.65-fold increase in Chrysosporine production. It is a potent radical scavenger and significantly uplifts some selected enzymatic antioxidant markers in peritoneal macrophage cells. Chrysosporine-fed rats have elevated counts of beneficial microflora of Lactobacillus sp. and less occurrence of putrefying Clostridium perfringens. Present outcomes suggest that Chrysosporine has a pharmaceutical interest as an exogenous antioxidant with prebiotic effectivities.