An Enzyme Free Electrochemical Assay Utilizing Graphene Oxide Sheets Decorated with Silver Nanoparticles for the Identification of Pseudomonas aeruginosa

This work describes the chronoamperometry deposition of Chitosan/silver nanocomposite and applied as platform for the development of immunoassay for the detection of Pseudomonas aeruginosa. The film was characterized by UV (Ultra visible spectroscopy), FTIR (Fourier transform infra red), Scanning Electron Microscopy (SEM), Energy-Dispersive X-ray spectroscopy (EDAX) and electrochemical detection methods. The electrochemical detection of Pseudomonas aeruginosa was done based on the Anti Ps tagged with silver nanoparticles decorated graphene oxide sheets which in turn binds to the antigen and the response was observed using Hydrogen peroxide (H₂O₂) in PB (Phosphate Buffer) electrolyte. From the results, the sensitivity range is from 10¹ to 10⁷ CFU/mL and LOD is calculated as 9.36 CFU/ml. The developed immunosensor also have high stability, reproducibility and reusability.