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<p>Though molecular studies have revealed the drug-transporter gene ABCC 11 in ceruminous secretions, its expression in the Indian population and its potential link to keratosis obturans remain unexamined. Moreover, existing research has relied exclusively on blood-derived RNA. This study therefore aims to detect ABCC 11 expression directly in ceruminous and keratotic debris and to evaluate any association between the two conditions. Patients with ear wax and keratosis were categorised as Group A and Group B respectively. Wax and keratosis samples were collected from respective groups and stored in -80 degree. From the collected samples, RNA isolation was done following which cDNA conversion was done and with the desired primers the expression of ABCC 11 gene was studied. Amongst the 30 wax and 20 keratosis samples studied, an equal gender distribution of 50% was seen in keratosis. 50% of the keratosis and 50% of the ear wax group had a mean age distribution of 22.5years and 40years respectively. This study establishes the presence of ABCC11 in both earwax and keratosis debris, with expression levels found to be 1.2 times higher in keratosis compared to earwax. Our findings confirmed that ABCC11 is expressed in glandular secretions, including both earwax and keratotic debris, and is not restricted to blood samples. Considering this gene’s role in drug transport and apocrine gland function, a simple earwax sample could potentially be used in the future to assess a patient’s response to chemotherapy and their risk of developing glandular tumours. Level 2.</p>

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Gene Expression Overlap Between Ear Wax and Keratosis Obturans: Insights into a Potential Pathogenic Link

  • 期刊论文

  • 发表日期：

    2025年9月10日

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  • Meykandanathan Aravinthan,
  • Selvarajan Gopal,
  • P. J. Shiny,
  • Subhashis Pal,
  • Malarvizhi Ravisankar,
  • C. R. K. Balaji

摘要

Though molecular studies have revealed the drug-transporter gene ABCC 11 in ceruminous secretions, its expression in the Indian population and its potential link to keratosis obturans remain unexamined. Moreover, existing research has relied exclusively on blood-derived RNA. This study therefore aims to detect ABCC 11 expression directly in ceruminous and keratotic debris and to evaluate any association between the two conditions. Patients with ear wax and keratosis were categorised as Group A and Group B respectively. Wax and keratosis samples were collected from respective groups and stored in -80 degree. From the collected samples, RNA isolation was done following which cDNA conversion was done and with the desired primers the expression of ABCC 11 gene was studied. Amongst the 30 wax and 20 keratosis samples studied, an equal gender distribution of 50% was seen in keratosis. 50% of the keratosis and 50% of the ear wax group had a mean age distribution of 22.5years and 40years respectively. This study establishes the presence of ABCC11 in both earwax and keratosis debris, with expression levels found to be 1.2 times higher in keratosis compared to earwax. Our findings confirmed that ABCC11 is expressed in glandular secretions, including both earwax and keratotic debris, and is not restricted to blood samples. Considering this gene’s role in drug transport and apocrine gland function, a simple earwax sample could potentially be used in the future to assess a patient’s response to chemotherapy and their risk of developing glandular tumours. Level 2.