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Aims <p>To investigate the relationship between NSD2 expression in parotid carcinoma tissues and patients’ clinical characteristics, and to explore the effects of siRNA-mediated NSD2 gene knockdown on the proliferation, apoptosis, and invasive capabilities of parotid carcinoma SACC-2 cells.</p> Materials and Methods <p>This study included specimens from a total of 59 patients with parotid carcinoma. SACC-2 cells were transfected with NSD2 siRNA. Real-time quantitative PCR was used to detect expression of NSD2 mRNA and western blot was used to detect the expression of NSD2 protein. CCK-8 and colony formation test were used to detect cell proliferation. Annexin V PI double staining flow cytometry was used to detect apoptosis, cell scratch and transwell test were used to detect cell invasion.</p> Results <p>The apoptosis rates of siNSD2-1 and siNSD2-2 groups were significantly higher. The wounds of siNSD2-1 and siNSD2-2 group were more significant than that of control, and relative migration distances were reduced significantly than that of control (<i>P</i> &lt; 0.05). The cells which penetrated cell membrane of siNSD2-1 and siNSD2-2 were significantly less than those of control (<i>P</i> &lt; 0.05).</p> Conclusions <p>Silencing NSD2 gene can inhibit the proliferation and invasion of parotid carcinoma SACC-2 cells and induce apoptosis.</p>

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The Expression and Regulation of the Nuclear Receptor-Binding SET Domain Protein 2 in Parotid Carcinoma

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月1日

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  • Min Gu,
  • Junyi Ge,
  • Qianjia Pan,
  • Chenyang Shi,
  • Peipei Wang,
  • Fei Hua

摘要

Aims

To investigate the relationship between NSD2 expression in parotid carcinoma tissues and patients’ clinical characteristics, and to explore the effects of siRNA-mediated NSD2 gene knockdown on the proliferation, apoptosis, and invasive capabilities of parotid carcinoma SACC-2 cells.

Materials and Methods

This study included specimens from a total of 59 patients with parotid carcinoma. SACC-2 cells were transfected with NSD2 siRNA. Real-time quantitative PCR was used to detect expression of NSD2 mRNA and western blot was used to detect the expression of NSD2 protein. CCK-8 and colony formation test were used to detect cell proliferation. Annexin V PI double staining flow cytometry was used to detect apoptosis, cell scratch and transwell test were used to detect cell invasion.

Results

The apoptosis rates of siNSD2-1 and siNSD2-2 groups were significantly higher. The wounds of siNSD2-1 and siNSD2-2 group were more significant than that of control, and relative migration distances were reduced significantly than that of control (P < 0.05). The cells which penetrated cell membrane of siNSD2-1 and siNSD2-2 were significantly less than those of control (P < 0.05).

Conclusions

Silencing NSD2 gene can inhibit the proliferation and invasion of parotid carcinoma SACC-2 cells and induce apoptosis.