Let \(X_r\) denote the blow-up of the hyperelliptic surface X at r very general points. In this paper, we first provide a criterion for the ampleness of a line bundle on \(X_r\) and compare it with existing results. We then study the multi-point Seshadri constants of ample line bundles on hyperelliptic surfaces X. Next, we compute single-point Seshadri constants on \(X_r\) for specific ample line bundles on odd types. Furthermore, we show that the global Seshadri constants for certain ample line bundles on blow-up of hyperelliptic surfaces are rational.