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Background <p>Cytochrome P450 proteins play essential roles in plant secondary metabolism and abiotic stress responses. As a drought-tolerant crop, <i>Sorghum bicolor</i> should be systematically analyzed to identify drought-responsive <i>P450</i> genes potentially useful for enhancing drought resistance via molecular breeding.</p> Results <p>Using transcriptome datasets and bioinformatics tools, we identified 33 drought-responsive <i>P450</i> genes (<i>SbP450-1</i> to <i>SbP450-33</i>) in sorghum on the basis of |log<sub>2</sub>(fold change)|≥ 1 and false discovery rate &lt; 0.05. These genes were distributed on 10 chromosomes and clustered into four families (<i>CYP71</i>, <i>CYP85</i>, <i>CYP72</i>, and <i>CYP86</i>). Notably, all <i>CYP86</i> family members lacked introns, while three pairs of tandem duplicates were detected. <i>SbP450</i> promoters contained many ABA/MeJA-responsive elements. Moreover, qRT-PCR analyses confirmed that <i>SbP450-29</i> and <i>SbP450-26</i> expression levels were significantly up-regulated after drought and ABA treatments (293- and 371-fold increases, respectively). Most SbP450 proteins were localized to chloroplasts, but SbP450-12 was detected in the plasma membrane and cytoplasm. GO/KEGG analyses linked SbP450s to flavonoid biosynthesis and redox pathways.</p> Conclusion <p>These findings provide a basis for future studies on the biological functions of sorghum <i>SbP450</i> genes.</p>

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Genome-Wide Identification of 33 Drought-Responsive P450 Genes in Sorghum Bicolor and Analyses of their Tissue-Specific Expression Under Abiotic Stress Conditions

  • 期刊论文

  • 发表日期：

    2026年6月26日

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  • Senjie Fu,
  • Junxia Li,
  • Na Qin,
  • Ya Jing,
  • Cancan Zhu,
  • Shutao Dai,
  • Chengyang Zhang,
  • Chunyi Wang

摘要

Background

Cytochrome P450 proteins play essential roles in plant secondary metabolism and abiotic stress responses. As a drought-tolerant crop, Sorghum bicolor should be systematically analyzed to identify drought-responsive P450 genes potentially useful for enhancing drought resistance via molecular breeding.

Results

Using transcriptome datasets and bioinformatics tools, we identified 33 drought-responsive P450 genes (SbP450-1 to SbP450-33) in sorghum on the basis of |log2(fold change)|≥ 1 and false discovery rate < 0.05. These genes were distributed on 10 chromosomes and clustered into four families (CYP71, CYP85, CYP72, and CYP86). Notably, all CYP86 family members lacked introns, while three pairs of tandem duplicates were detected. SbP450 promoters contained many ABA/MeJA-responsive elements. Moreover, qRT-PCR analyses confirmed that SbP450-29 and SbP450-26 expression levels were significantly up-regulated after drought and ABA treatments (293- and 371-fold increases, respectively). Most SbP450 proteins were localized to chloroplasts, but SbP450-12 was detected in the plasma membrane and cytoplasm. GO/KEGG analyses linked SbP450s to flavonoid biosynthesis and redox pathways.

Conclusion

These findings provide a basis for future studies on the biological functions of sorghum SbP450 genes.