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<p>Pre-harvest sprouting (PHS) in greengram is a complex phenomenon that is influenced by both genetic and environmental factors, resulting in major yield losses. Identification of molecular markers is essential for development of PHS tolerance greengram varieties through marker-assisted breeding. This study was undertaken to identify molecular markers associated with PHS tolerance through Bulked Line Analysis (BLA).&#xa0;The phenotypic evaluation of 83 greengram genotypes for PHS, and its related seed, and pod traits under controlled laboratory conditions revealed four tolerant genotypes viz., LGG 633, LGG 607, PUSA M 19,111, MGG 385 with less than 10% PHS. This finding is further supported by the fact that PHS-tolerant genotypes exhibited a slower increase rate of alpha-amylase activity than susceptible ones, particularly at 48&#xa0;h after germination. Correlation analysis revealed a strong positive association of PHS with water imbibition by seed, and fresh seed germination while a significant negative correlation with epicuticular wax content. The bulked line analysis for three traits, namely PHS, pod wall thickness, and epicuticular wax content revealed that the CEDG228 marker has shown a strong association with pre-harvest sprouting tolerance.&#xa0;The marker CEDG228 identified in the present study has great potential for marker-assisted breeding facilitating the development of PHS-tolerant green gram varieties.</p>

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Identification of a Potential Molecular Marker for Pre-Harvest Sprouting Tolerance in Greengram (Vigna Radiata (L.) Wilczek)

  • 期刊论文

  • 发表日期：

    2026年2月5日

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  • Venkataraja V. Mokshith,
  • Veeraghattapu Roja,
  • Namburi Harisatyanarayana,
  • Purru Supriya,
  • Koppolu Jayalalitha,
  • Jallu Pranaya,
  • Pandiri J. S. Pramod,
  • Galla Geethanjali,
  • Sudhamani Kalluru,
  • Lakshminarayana R. Vemireddy

摘要

Pre-harvest sprouting (PHS) in greengram is a complex phenomenon that is influenced by both genetic and environmental factors, resulting in major yield losses. Identification of molecular markers is essential for development of PHS tolerance greengram varieties through marker-assisted breeding. This study was undertaken to identify molecular markers associated with PHS tolerance through Bulked Line Analysis (BLA). The phenotypic evaluation of 83 greengram genotypes for PHS, and its related seed, and pod traits under controlled laboratory conditions revealed four tolerant genotypes viz., LGG 633, LGG 607, PUSA M 19,111, MGG 385 with less than 10% PHS. This finding is further supported by the fact that PHS-tolerant genotypes exhibited a slower increase rate of alpha-amylase activity than susceptible ones, particularly at 48 h after germination. Correlation analysis revealed a strong positive association of PHS with water imbibition by seed, and fresh seed germination while a significant negative correlation with epicuticular wax content. The bulked line analysis for three traits, namely PHS, pod wall thickness, and epicuticular wax content revealed that the CEDG228 marker has shown a strong association with pre-harvest sprouting tolerance. The marker CEDG228 identified in the present study has great potential for marker-assisted breeding facilitating the development of PHS-tolerant green gram varieties.