<p>Sulfotransferases (SOTs) are sulfate-regulating proteins that playing crucial roles in plant growth, development, and abiotic stress response. However, the role of SOTs in rice under pesticide stress is yet unknown. To improve our understanding of how rice SOTs are affected by pesticide-induced stress, we herein examined 23 and 27 SOT-coding genes from the transcriptomic datasets of oxyfluorfen (OFF)- and fluroxypyr-meptyl (FLUME)-treated rice, respectively, and identified two and three SOT-differentially expressed genes (DEGs) in them, respectively. Comprehensive bioinformatics analyses were conducted to characterize their evolutionary relationships, silico subcellular localization, gene structures, promoter cis-elements, and expression patterns. In silico subcellular localization prediction suggested that these DEGs coding SOTs are mainly localized in the cytoplasm. A variety of conserved domains, <i>cis</i>-elements, motif compositions, and gene architectures were also present in these genes, enabling them to encode different proteins implicated in abiotic stress responses. Next, controlled experiments showed that SOT activity in rice tissue was stimulated during 0.07–0.23 mg L^− 1 OFF and 0.02–0.14 mg L^− 1 FLUME stress, these levels increased by 1.7 and 1.3-fold compared with those in control, respectively. Finally, OsSOT proteins were implicated in OFF and FLUME metabolism, according to a network study of protein–protein interactions. The preliminary results of this investigation delineate the characteristics of the OsSOT superfamily and provide crucial instruments for functional evaluations of <i>OsSOT</i> genes linked to the metabolism of OFF and FLUME.</p>