Scholarlink

<p>Species of the genus <i>Wittmackia</i> have high potential for the ornamental plant market due to their diverse shapes, colors, and ecological adaptations. These characteristics make them promising for genetic improvement programs aimed at creating new hybrids. However, the asynchrony of flowering in this genus limits the simultaneous availability of flowers, making controlled crosses difficult. Cryopreservation of pollen is an important technique to conserve alleles and overcome parental flowering asynchrony. This study evaluates the feasibility and functionality of cryopreservation of <i>Wittmackia</i> pollen grains for the conservation of genetic resources and interspecific hybridization. The pollen grains were dehydrated in silica gel for three hours, and for in vitro germination, they were inoculated in SM medium (Soares et al. <CitationRef CitationID="CR61">2008</CitationRef>). The grains were cryopreserved for 30, 180 and 360 days. For the in vivo viability test, controlled crosses were performed to measure the production of fruits and seeds, and the growth of pollen tube in the pistil. Pollen dehydration was effective in 87% of the species, with water content ranging from 20.00% to 31.02%, except for <i>W. limae</i> and <i>W. linharesiorum</i>. The percentage of viable grains after cryopreservation varied from 14.96% in <i>W. linharesiorum</i> to 98.78% in <i>W. laevigata</i>, and pollen tube length ranged from 0.25 ± 0.02&#xa0;mm in <i>W. linharesiorum</i> to 1.12 ± 0.07&#xa0;mm in <i>W. limae</i>. In total, 713 pollinations were carried out, 17% by xenogamy and 83% by interspecific hybridization in 71 combinations. Success was achieved in 37% of the pollinations by xenogamy and 63% of the interspecific hybridizations. All xenogamous pollinations resulted in fruits, with 8 to 285 seeds each. Lack of germination, pollen tube coiling, and failure to penetrate the micropyle were observed in some cases. In vitro seed germination produced normal seedlings that are now being used for in vitro conservation studies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Reproductive Potential of Cryopreserved Pollen in Wittmackia (Bromeliaceae): Implications for Conservation and Plant Breeding

  • 期刊论文

  • 发表日期：

    2025年11月17日

查看全文
  • Gleice Quelle Silva Santos dos Nascimento,
  • Fernanda Vidigal Duarte Souza,
  • Lidyanne Yuriko Saleme Aona,
  • Maria Angélica Pereira de Carvalho Costa,
  • Everton Hilo de Souza

摘要

Species of the genus Wittmackia have high potential for the ornamental plant market due to their diverse shapes, colors, and ecological adaptations. These characteristics make them promising for genetic improvement programs aimed at creating new hybrids. However, the asynchrony of flowering in this genus limits the simultaneous availability of flowers, making controlled crosses difficult. Cryopreservation of pollen is an important technique to conserve alleles and overcome parental flowering asynchrony. This study evaluates the feasibility and functionality of cryopreservation of Wittmackia pollen grains for the conservation of genetic resources and interspecific hybridization. The pollen grains were dehydrated in silica gel for three hours, and for in vitro germination, they were inoculated in SM medium (Soares et al. 2008). The grains were cryopreserved for 30, 180 and 360 days. For the in vivo viability test, controlled crosses were performed to measure the production of fruits and seeds, and the growth of pollen tube in the pistil. Pollen dehydration was effective in 87% of the species, with water content ranging from 20.00% to 31.02%, except for W. limae and W. linharesiorum. The percentage of viable grains after cryopreservation varied from 14.96% in W. linharesiorum to 98.78% in W. laevigata, and pollen tube length ranged from 0.25 ± 0.02 mm in W. linharesiorum to 1.12 ± 0.07 mm in W. limae. In total, 713 pollinations were carried out, 17% by xenogamy and 83% by interspecific hybridization in 71 combinations. Success was achieved in 37% of the pollinations by xenogamy and 63% of the interspecific hybridizations. All xenogamous pollinations resulted in fruits, with 8 to 285 seeds each. Lack of germination, pollen tube coiling, and failure to penetrate the micropyle were observed in some cases. In vitro seed germination produced normal seedlings that are now being used for in vitro conservation studies.