Scholarlink

<p>Studies focused on identifying and characterizing genetic resources with potential for productivity, adaptability and resistance to biotic and abiotic stresses are relevant for better understanding passion fruit tree crops. In this work, intra- and interspecific genetic diversity was evaluated on the basis of <i>loci</i> analogous to resistance genes in wild and cultivated species of the genus <i>Passiflora</i>. A total of 56 <i>Passiflora</i> species were analyzed in this study using arbitrary primers targeting the resistance gene analog (RGA) domain, including those previously designed for <i>P. alata</i> and species of other genera: <i>Glycine max</i>, <i>Arabidopsis thaliana</i>, <i>Nicotiana tabacum</i> and <i>Linum usitatissimum</i>. Successful amplification with both primer sets revealed a high level of inter- and intraspecific genetic diversity within the studied collection. On the basis of the Jaccard similarity coefficient, the species of greatest commercial relevance, <i>P. edulis</i> and <i>P. alata</i>, were clustered into separate groups, revealing a large genetic distance for genetic regions associated with disease resistance. These findings provide valuable information for designing inter- and intraspecific crosses aimed at generating segregating populations and facilitating the introgression of resistance traits into commercial <i>Passiflora</i> species.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Analysis of Genetic Diversity in Passiflora Revealed by Resistance Gene Analog (RGA) Markers

  • 期刊论文

  • 发表日期：

    2025年7月10日

查看全文
  • Ohana Luíza Santos de Oliveira,
  • Cláusio Antônio Ferreira de Melo,
  • Margarete Magalhães Souza

摘要

Studies focused on identifying and characterizing genetic resources with potential for productivity, adaptability and resistance to biotic and abiotic stresses are relevant for better understanding passion fruit tree crops. In this work, intra- and interspecific genetic diversity was evaluated on the basis of loci analogous to resistance genes in wild and cultivated species of the genus Passiflora. A total of 56 Passiflora species were analyzed in this study using arbitrary primers targeting the resistance gene analog (RGA) domain, including those previously designed for P. alata and species of other genera: Glycine max, Arabidopsis thaliana, Nicotiana tabacum and Linum usitatissimum. Successful amplification with both primer sets revealed a high level of inter- and intraspecific genetic diversity within the studied collection. On the basis of the Jaccard similarity coefficient, the species of greatest commercial relevance, P. edulis and P. alata, were clustered into separate groups, revealing a large genetic distance for genetic regions associated with disease resistance. These findings provide valuable information for designing inter- and intraspecific crosses aimed at generating segregating populations and facilitating the introgression of resistance traits into commercial Passiflora species.