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<p>β-ketoacyl-CoA synthase (KCS) is a crucial rate-limiting enzyme in the synthesis of very-long-chain fatty acids (VLCFAs), primarily responsible for regulating the synthesis and accumulation of fatty acids and waxes. Although <i>KCSs</i> have been identified in various plants, a comprehensive genomic analysis of <i>KCSs</i> in castor (<i>Ricinus communis</i> L.) has not been reported. In this study, we identified <i>RcKCSs</i> in the castor genome through sequence alignment and analyzed their gene structure, protein motifs, physicochemical properties, transcription patterns under cold stress, and expression patterns across different tissues. A total of 17 <i>RcKCSs</i> were identified in the castor genome. Phylogenetic analysis classified the RcKCSs into 12 subgroups, suggesting that distinct RcKCSs may have different biological functions. Additionally, several abiotic stress and hormone response <i>cis</i>-acting elements were identified in the promoters of <i>RcKCSs</i>. RNA-seq analysis revealed that <i>RcKCS1</i>, <i>RcKCS5</i>, and <i>RcKCS14</i> were induced by cold stress and showed upregulation over time, indicating their involvement in the regulation of cold adaptation in castor plants. The expression of <i>RcKCSs</i> varied across different developmental stages of castor, with higher levels observed in male flowers and leaves. This study provides valuable insights into the potential roles of <i>RcKCSs</i> in regulating the growth and development of castor and its response to cold stress.</p>

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Identification and Expression Pattern Analysis of the β-Ketoacyl-CoA Synthase (KCS) Gene Family in Castor (Ricinus communis L.)

  • 期刊论文

  • 发表日期：

    2025年2月25日

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  • Yuan Zhang,
  • Xue Wei,
  • Hongyan Huo,
  • Nan Wang,
  • Xue Ding,
  • Lili Yu,
  • WenRan Yue,
  • Hui Xu,
  • Xiumin Yu,
  • Yan Liu,
  • Gaowa Xilin,
  • Jixing Zhang,
  • Xiaoyu Wang

摘要

β-ketoacyl-CoA synthase (KCS) is a crucial rate-limiting enzyme in the synthesis of very-long-chain fatty acids (VLCFAs), primarily responsible for regulating the synthesis and accumulation of fatty acids and waxes. Although KCSs have been identified in various plants, a comprehensive genomic analysis of KCSs in castor (Ricinus communis L.) has not been reported. In this study, we identified RcKCSs in the castor genome through sequence alignment and analyzed their gene structure, protein motifs, physicochemical properties, transcription patterns under cold stress, and expression patterns across different tissues. A total of 17 RcKCSs were identified in the castor genome. Phylogenetic analysis classified the RcKCSs into 12 subgroups, suggesting that distinct RcKCSs may have different biological functions. Additionally, several abiotic stress and hormone response cis-acting elements were identified in the promoters of RcKCSs. RNA-seq analysis revealed that RcKCS1, RcKCS5, and RcKCS14 were induced by cold stress and showed upregulation over time, indicating their involvement in the regulation of cold adaptation in castor plants. The expression of RcKCSs varied across different developmental stages of castor, with higher levels observed in male flowers and leaves. This study provides valuable insights into the potential roles of RcKCSs in regulating the growth and development of castor and its response to cold stress.