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<p>Quorum sensing (QS) is a critical communication system in bacteria that enables them to sense population density through auto-inducers, notably, <i>N-</i>acyl homoserine lactones (AHLs). This study focuses on the role of QS in&#xa0;<i>Vibrio parahaemolyticus</i>, a significant pathogen affecting vannamei shrimp, where it regulates virulence factors such as hemolysins and biofilm formation. We explored the potential of anti-QS agents derived from&#xa0;a <i>Psychrobacter</i> sp., which produces AHL lactonase capable of degrading AHLs and disrupting QS signaling pathways. The purification of AHL lactonase was achieved through chromatographic techniques, yielding a protein with a molecular weight of 28–30 kDa, consistent with previous findings. AHL degradation tests have demonstrated that AHL lactonase was significantly more effective than metabolites from&#xa0;<i>Psychrobacter</i>&#xa0;in breaking down AHLs. However, the metabolites exhibited superior biofilm inhibition activity, indicating distinct mechanisms in disrupting bacterial adherence and virulence. Gene expression analysis revealed that AHL lactonase effectively suppressed virulence-related genes, while the metabolites influenced gene regulation differently. This study highlights the dual mechanisms of action from&#xa0;a <i>Psychrobacter</i> sp. – direct AHL degradation and inhibition of AHL synthesis, offering a promising biocontrol strategy against&#xa0;<i>V. parahaemolyticus</i> infections in shrimp aquaculture. These findings suggest that integrating these approaches could enhance pathogen management, ultimately supporting sustainable aquaculture practices. Further research is warranted to elucidate the underlying molecular mechanisms and optimize the application of these biocontrol agents in disease management.</p>

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Exploring Psychrobacter sp. EQ05 (bacterial symbiont of Entaqmea quadricolor): A natural anti-quorum-sensing agent against Vibrio parahaemolyticus

  • 期刊论文

  • 发表日期：

    2026年1月21日

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  • Baso Manguntungi,
  • Iman Rusmana,
  • Apon Zaenal Mustopa,
  • Anja Meryandini

摘要

Quorum sensing (QS) is a critical communication system in bacteria that enables them to sense population density through auto-inducers, notably, N-acyl homoserine lactones (AHLs). This study focuses on the role of QS in Vibrio parahaemolyticus, a significant pathogen affecting vannamei shrimp, where it regulates virulence factors such as hemolysins and biofilm formation. We explored the potential of anti-QS agents derived from a Psychrobacter sp., which produces AHL lactonase capable of degrading AHLs and disrupting QS signaling pathways. The purification of AHL lactonase was achieved through chromatographic techniques, yielding a protein with a molecular weight of 28–30 kDa, consistent with previous findings. AHL degradation tests have demonstrated that AHL lactonase was significantly more effective than metabolites from Psychrobacter in breaking down AHLs. However, the metabolites exhibited superior biofilm inhibition activity, indicating distinct mechanisms in disrupting bacterial adherence and virulence. Gene expression analysis revealed that AHL lactonase effectively suppressed virulence-related genes, while the metabolites influenced gene regulation differently. This study highlights the dual mechanisms of action from a Psychrobacter sp. – direct AHL degradation and inhibition of AHL synthesis, offering a promising biocontrol strategy against V. parahaemolyticus infections in shrimp aquaculture. These findings suggest that integrating these approaches could enhance pathogen management, ultimately supporting sustainable aquaculture practices. Further research is warranted to elucidate the underlying molecular mechanisms and optimize the application of these biocontrol agents in disease management.