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<p>SCRBQ2 is a croquemort-scavenger receptor class B family protein. The identification of SCRBQ2 in the detergent-resistant membrane of the <i>Anopheles stephensi</i> midgut suggests its role as an ookinete-interacting protein, positioning it as a potential target for transmission-blocking vaccine development. The <i>Asscrbq2</i> gene was characterized in the Indian urban malaria vector <i>A. stephensi</i>. It was amplified and submitted to GenBank with accession ID ON817188. The gene consists of a coding sequence (CDS) of 2922&#xa0;bp and encodes a protein of 973 amino acids. The AsSCRBQ2 protein contains two merged CD36 domains, which are conserved across various mosquito species. A similar evolutionary merging of these domains has also been observed in <i>Anopheles culicifacies</i>, <i>Anopheles maculipennis</i>, <i>Anopheles minimus</i>, <i>Anopheles dirus</i>, and <i>Anopheles aquasalis</i>. AsSCRBQ2 domain I shows 91.02% similarity with SCRBQ1 of <i>Anopheles gambiae</i>, whereas domain II shows 88.68% similarity with SCRBQ2 of <i>A. gambiae</i>. The similarity between human CD36 and AsSCRBQ2 highlights the functional versatility of AsSCRBQ2 in mediating immune responses and facilitating <i>Plasmodium falciparum</i> transmission in malaria vectors. Its dual interactions with PfEMP1 and TLR4 position AsSCRBQ2 as a promising target for transmission-blocking vaccine development and vector control strategies. Codon optimization and <i>in silico</i> cloning showed that the <i>Asscrbq2</i> gene is highly expressed in the <i>Escherichia coli</i> strain K12. The relative expression pattern shows that the <i>Asscrbq2</i> gene is highly expressed in the midgut than in the fat body, followed by the salivary glands and ovaries. After blood feeding, upregulation was observed in the midgut at 24&#xa0;h post blood meal (PBM), suggesting a role in blood digestion, and in the fat body at 48&#xa0;h PBM, indicating a possible role in immunity. Previous studies have suggested a role for SCRBQ2 in <i>Plasmodium</i> invasion of the mosquito midgut; however, this remains to be investigated and validated specifically in <i>A. stephensi</i>.</p> Graphical abstract <p></p>

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Croquemort-scavenger receptor class B 2 (scrbq2) gene in Anopheles stephensi: Molecular characterization and expression analysis after blood feeding

  • 期刊论文

  • 发表日期：

    2025年11月10日

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  • Nisha Dahiya,
  • Mahima Yadav,
  • Vartika Srivastava,
  • Sangeeta Janjoter,
  • Divya Kataria,
  • Rajnikant Dixit,
  • Neelam Sehrawat

摘要

SCRBQ2 is a croquemort-scavenger receptor class B family protein. The identification of SCRBQ2 in the detergent-resistant membrane of the Anopheles stephensi midgut suggests its role as an ookinete-interacting protein, positioning it as a potential target for transmission-blocking vaccine development. The Asscrbq2 gene was characterized in the Indian urban malaria vector A. stephensi. It was amplified and submitted to GenBank with accession ID ON817188. The gene consists of a coding sequence (CDS) of 2922 bp and encodes a protein of 973 amino acids. The AsSCRBQ2 protein contains two merged CD36 domains, which are conserved across various mosquito species. A similar evolutionary merging of these domains has also been observed in Anopheles culicifacies, Anopheles maculipennis, Anopheles minimus, Anopheles dirus, and Anopheles aquasalis. AsSCRBQ2 domain I shows 91.02% similarity with SCRBQ1 of Anopheles gambiae, whereas domain II shows 88.68% similarity with SCRBQ2 of A. gambiae. The similarity between human CD36 and AsSCRBQ2 highlights the functional versatility of AsSCRBQ2 in mediating immune responses and facilitating Plasmodium falciparum transmission in malaria vectors. Its dual interactions with PfEMP1 and TLR4 position AsSCRBQ2 as a promising target for transmission-blocking vaccine development and vector control strategies. Codon optimization and in silico cloning showed that the Asscrbq2 gene is highly expressed in the Escherichia coli strain K12. The relative expression pattern shows that the Asscrbq2 gene is highly expressed in the midgut than in the fat body, followed by the salivary glands and ovaries. After blood feeding, upregulation was observed in the midgut at 24 h post blood meal (PBM), suggesting a role in blood digestion, and in the fat body at 48 h PBM, indicating a possible role in immunity. Previous studies have suggested a role for SCRBQ2 in Plasmodium invasion of the mosquito midgut; however, this remains to be investigated and validated specifically in A. stephensi.

Graphical abstract