Scholarlink

<p>Parkinson’s disease (PD) is the second most prevalent neurodegenerative disease. With the intensification of population aging, its incidence rate continues to rise. Microglia play a significant role in regulating neuronal functions. As carriers of intercellular communication, exosomes can mediate intercellular information transmission and signal regulation. This study aimed to explore the molecular mechanism by which <i>Acanthopanax senticosus</i> extract (AS) interferes with the PD process by regulating BV2 microglial cells exosome communication. A PD-related inflammation model was established by inducing BV2 microglial cells activation with lipopolysaccharide (LPS). Exosomes derived from microglia were isolated by ultracentrifugation. The exosomes were co-cultured with the SHSY5Y-flag-snca cells to detect the expression levels of <i>α</i>-synuclein(<i>α</i>-syn) and tyrosine hydroxylase (TH) in these cells. Transcriptome sequencing using exosomes revealed that the mechanism by which AS treats PD involves the regulation of the long noncoding RNA–microRNA–messenger RNA network in exosomes, and the expression of target genes differentially expressed by exosomes was verified by the RT-qPCR method. This study explored at the molecular level the mechanism by which AS regulates BV2 microglial cells exosomes in intercellular communication therapy for PD, providing new research directions and theoretical basis for finding therapeutic targets for PD.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Study on the Mechanism of Acanthopanax senticosus Extract Intervening in the Regulation of Parkinson’s Disease by BV2 Microglial Cell–Derived Exosomes

  • 期刊论文

  • 发表日期：

    2025年11月24日

查看全文
  • Ningxia Lu,
  • Yehao Wang,
  • Yi Lu,
  • Fang Lu,
  • Shumin Liu

摘要

Parkinson’s disease (PD) is the second most prevalent neurodegenerative disease. With the intensification of population aging, its incidence rate continues to rise. Microglia play a significant role in regulating neuronal functions. As carriers of intercellular communication, exosomes can mediate intercellular information transmission and signal regulation. This study aimed to explore the molecular mechanism by which Acanthopanax senticosus extract (AS) interferes with the PD process by regulating BV2 microglial cells exosome communication. A PD-related inflammation model was established by inducing BV2 microglial cells activation with lipopolysaccharide (LPS). Exosomes derived from microglia were isolated by ultracentrifugation. The exosomes were co-cultured with the SHSY5Y-flag-snca cells to detect the expression levels of α-synuclein(α-syn) and tyrosine hydroxylase (TH) in these cells. Transcriptome sequencing using exosomes revealed that the mechanism by which AS treats PD involves the regulation of the long noncoding RNA–microRNA–messenger RNA network in exosomes, and the expression of target genes differentially expressed by exosomes was verified by the RT-qPCR method. This study explored at the molecular level the mechanism by which AS regulates BV2 microglial cells exosomes in intercellular communication therapy for PD, providing new research directions and theoretical basis for finding therapeutic targets for PD.