Scholarlink

<p>The Gram-positive, filamentous soil bacterium <i>Streptomyces mirabilis</i> P16B-1 has been shown to tolerate high environmental concentrations of Ni. The extremely high metal tolerance is partly provided through biomineralization, observed for both Mg bearing struvite and Ni-struvite where Ni ions replace Mg in the mineral lattice. The minerals showed different morphologies. This led us to assume that excreted substances may have the capacity to influence the mineral formation process that occurs at a distance to the cells. Here, we could show with metabolomics and proteomics studies that 23 metabolites as well as 18 proteins potentially co-precipitate with the minerals. Secreted secondary metabolites reduced in the supernatant after mineralization were identified as ergothioneine, diacetyllegionaminic acid, nevaltophin F, and diaminonaphthalene. The compounds phenylacetaldehyde and margaric acid also reduced after mineral precipitation did not alter mineral macromorphology. In addition, ten proteins that co-precipitated with statistical significance were predicted to be secreted. Among those, a specific nickel binding protein, NikA, was reduced in high amounts from the supernatant upon crystal formation. The potential of secreted organic molecules and proteins to bind specific surfaces of the mineral thereby redirecting crystal growth in a microbially influenced biomineralization process. Implications for biotechnological metal-struvite use are discussed.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Organic Molecules are Involved in Ni-Struvite Biomineralization by Streptomyces mirabilis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月15日

查看全文
  • Flávio Silva Costa,
  • Thomas Krüger,
  • Nico Ueberschaar,
  • Olaf Kniemeyer,
  • Axel Brakhage,
  • Erika Kothe

摘要

The Gram-positive, filamentous soil bacterium Streptomyces mirabilis P16B-1 has been shown to tolerate high environmental concentrations of Ni. The extremely high metal tolerance is partly provided through biomineralization, observed for both Mg bearing struvite and Ni-struvite where Ni ions replace Mg in the mineral lattice. The minerals showed different morphologies. This led us to assume that excreted substances may have the capacity to influence the mineral formation process that occurs at a distance to the cells. Here, we could show with metabolomics and proteomics studies that 23 metabolites as well as 18 proteins potentially co-precipitate with the minerals. Secreted secondary metabolites reduced in the supernatant after mineralization were identified as ergothioneine, diacetyllegionaminic acid, nevaltophin F, and diaminonaphthalene. The compounds phenylacetaldehyde and margaric acid also reduced after mineral precipitation did not alter mineral macromorphology. In addition, ten proteins that co-precipitated with statistical significance were predicted to be secreted. Among those, a specific nickel binding protein, NikA, was reduced in high amounts from the supernatant upon crystal formation. The potential of secreted organic molecules and proteins to bind specific surfaces of the mineral thereby redirecting crystal growth in a microbially influenced biomineralization process. Implications for biotechnological metal-struvite use are discussed.