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<p>The evaluation of disease progression in rats with severe pneumonia and septic shock relies on the expression of indicators in the lung, intestinal microenvironment, and serum. A rat model was created for this purpose and the serum samples were collected at various stages. The levels of procalcitonin (PCT), C-reactive protein (CRP), surfactant protein A (SP-A), and tumor necrosis factor α (TNF-α) in the serum and SP-A in the alveolar lavage fluid were measured using enzyme-linked immunosorbent assay ELISA. Additionally, western blot was used to measure SP-A and TNF-α levels in lung and intestinal tissues. The results indicated that early in severe pneumonia, intestinal damage was less than lung damage, but it worsened over time. SP-A expression in intestinal tissue rose within 12&#xa0;h post-modeling and matched lung tissue after 24&#xa0;h, showing a declining trend. After modeling, the serum levels of SP-A and TNF-α were significantly higher than the control group. SP-A can indicate the health of the blood gas barriers and intestinal mucosal barriers. Tracking TNF-α and SP-A levels during severe pneumonia can offer precise data on disease progression and prognosis.</p>

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Interplay of Surfactant Protein A and Tumor Necrosis Factor α in Lung and Intestinal Tissues of Rats with Severe Pneumonia

  • 期刊论文

  • 发表日期：

    2025年4月24日

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  • Jin Zhang,
  • Yangjia Deng,
  • Guansheng Li,
  • Xiaoping Sun

摘要

The evaluation of disease progression in rats with severe pneumonia and septic shock relies on the expression of indicators in the lung, intestinal microenvironment, and serum. A rat model was created for this purpose and the serum samples were collected at various stages. The levels of procalcitonin (PCT), C-reactive protein (CRP), surfactant protein A (SP-A), and tumor necrosis factor α (TNF-α) in the serum and SP-A in the alveolar lavage fluid were measured using enzyme-linked immunosorbent assay ELISA. Additionally, western blot was used to measure SP-A and TNF-α levels in lung and intestinal tissues. The results indicated that early in severe pneumonia, intestinal damage was less than lung damage, but it worsened over time. SP-A expression in intestinal tissue rose within 12 h post-modeling and matched lung tissue after 24 h, showing a declining trend. After modeling, the serum levels of SP-A and TNF-α were significantly higher than the control group. SP-A can indicate the health of the blood gas barriers and intestinal mucosal barriers. Tracking TNF-α and SP-A levels during severe pneumonia can offer precise data on disease progression and prognosis.