<p>Microbial carbonate mineralization, the process by which microorganisms (<i>Bacillus</i> sp., <i>Sporosarcina</i> sp., <i>Penicillium</i> sp., <i>Cyanobacteria</i>, etc.) directly mediate or indirectly influence mineral formation and deposition, represents the next frontier in technology with vast potential across scientific disciplines, including construction, environmental remediation, and carbon sequestration. This review explores the fundamental aspects of microbial carbonate mineralization, focusing on key mechanisms such as photosynthesis, methane oxidation, sulfate reduction, ureolysis, denitrification, carbonic anhydrase activity, iron reduction, and EPS mediation, all of which influence carbonate saturation and mineral nucleation. Additionally, it highlights critical regulatory factors that enhance biomineralization for bio-inspired material development in heavy metal remediation, wastewater treatment, self-healing concrete, biomedical applications, nanoscale technologies, and 3D printing. A major focus is microbial-induced calcite precipitation (MICP), an emerging and cost-efficient biomineralization technique, with an in-depth analysis of its molecular mechanisms and expanding applications. Furthermore, this review discusses current challenges, including process scalability, long-term stability, and environmental and safety considerations, while identifying future research directions to improve the efficacy and sustainability of microbial carbonate mineralization in advanced technological applications.</p> Graphical Abstract <p></p>