Scholarlink

<p>This cross-sectional study explored the potential utility of salivary circadian gene expression as a non-invasive biomarker for early cognitive impairment in shift workers. Three hundred participants aged 25 to 55 were categorized into cognitively impaired shift workers (MoCA &lt; 26, <i>n</i> = 100), cognitively intact shift workers (<i>n</i> = 100), and non-shift working controls (<i>n</i> = 100). Saliva samples collected at 07:00 and 19:00 were analyzed for mRNA expression of <i>PER1</i>, <i>BMAL1</i>, and <i>CLOCK</i> using qRT-PCR. Shift workers with cognitive impairment showed significantly attenuated diurnal variation in gene expression, with reduced evening levels of <i>BMAL1</i> and <i>PER1</i> compared to both control groups (<i>p</i> &lt; 0.001). Evening <i>BMAL1</i> expression was independently associated with cognitive status (OR 2.14, 95% CI 1.62–2.85), achieving an AUC of 0.876 (81.3% sensitivity, 78.0% specificity). A combined three-gene panel modestly improved diagnostic accuracy (AUC 0.913). These preliminary findings suggest that alterations in salivary circadian gene expression, particularly in <i>BMAL1</i>, may hold promise as a molecular indicator of early neurocognitive changes in shift-working populations.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Salivary Circadian Gene Expression as a Molecular Indicator of Early Cognitive Impairment in Shift Workers: A Cross-Sectional Study of 300 Adults

  • 期刊论文

  • 发表日期：

    2025年9月23日

查看全文
  • Adam Karim,
  • Youssef Rayan,
  • Ziad Amir,
  • Malik Samir,
  • Tariq Jamal,
  • Karim Adnan,
  • Omar Fares,
  • Kareem Zane,
  • Samir Tarek,
  • Amir Nasir,
  • Rami Khalid,
  • Zeyad Khalil,
  • Yasin Rafiq,
  • Idris Nader

摘要

This cross-sectional study explored the potential utility of salivary circadian gene expression as a non-invasive biomarker for early cognitive impairment in shift workers. Three hundred participants aged 25 to 55 were categorized into cognitively impaired shift workers (MoCA < 26, n = 100), cognitively intact shift workers (n = 100), and non-shift working controls (n = 100). Saliva samples collected at 07:00 and 19:00 were analyzed for mRNA expression of PER1, BMAL1, and CLOCK using qRT-PCR. Shift workers with cognitive impairment showed significantly attenuated diurnal variation in gene expression, with reduced evening levels of BMAL1 and PER1 compared to both control groups (p < 0.001). Evening BMAL1 expression was independently associated with cognitive status (OR 2.14, 95% CI 1.62–2.85), achieving an AUC of 0.876 (81.3% sensitivity, 78.0% specificity). A combined three-gene panel modestly improved diagnostic accuracy (AUC 0.913). These preliminary findings suggest that alterations in salivary circadian gene expression, particularly in BMAL1, may hold promise as a molecular indicator of early neurocognitive changes in shift-working populations.