Expression of Interleukin-8, Interleukin-12 and Interleukin-13 in Esophageal Squamous Cell Carcinoma: Biomarker Potentiality and Prognostic Significance

期刊论文
发表日期：

2024年6月23日

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Jayasree Talukdar,
Abdul Malik,
Kangkana Kataki,
Bikash Narayan Choudhury,
Munindra Narayan Baruah,
Mallika Bhattacharyya,
Manash Pratim Sarma,
Minakshi Bhattacharjee,
Mrinmoy Basak,
Manash Pratim Kashyap,
Sahana Bhattacharjee,
Eyashin Ali,
Chenole Keppen,
Simanta Kalita,
Manash Jyoti Kalita,
Partha Pratim Das,
Gautam Hazarika,
Ankur Jyoti Deka,
Kalpajit Dutta,
Mohammad Ghaznavi Idris,
Suhail Akhtar,
Subhash Medhi

摘要

Purpose

Interleukin-8 (IL8), Interleukin-12 (IL12) and Interleukin-13 (IL13) are cytokines that play regulatory role in cancer pathogenesis. We analysed their expression profile to evaluate as molecular biomarkers of esophageal squamous cell carcinoma (ESCC) and their association with different parameters and patient survival.

Methods

Expression analysis was performed by Real time quantitative polymerase chain reaction and receiver operating characteristic (ROC) curve analysis was done. The expression profiles were associated with different clinicopathological and dietary factors. Survival and hazard analysis were also performed.

Results

IL8 expression showed upregulation in tissue (p = 0.000) and blood samples (p = 0.481), IL12 expression showed downregulation in tissue samples (p = 0.064) and upregulation in blood samples (p = 0.689) and IL13 expression showed upregulation in tissue (p = 0.000) and blood samples (p = 0.006). IL13 expression in tissue showed the highest area under the curve (AUC) value (0.773) for ESCC diagnosis, followed by IL8 expression in tissue (0.704) and IL13 expression in blood (0.643). This study also reveals the correlation of studied cytokines in tissue and blood level. Different clinicopathological and dietary factors showed significant association (p < 0.05) with IL8, IL12 and IL13 expression and with survival of ESCC patients. IL8 expression in blood and IL12 expression in tissue and blood showed significant association (p < 0.05) with patient survival.

Conclusion

Altered expression of IL8, IL12 and IL13 may be associated with ESCC progression. Overexpression of IL8 and IL13 in tissue samples may be potential biomarkers for ESCC screening. Additionally, both survival and hazard analysis data indicate the effects of different parameters on the prognosis of ESCC patients.