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Purpose <p>The vasculature and sympathetic nervous system are critical for adrenal homeostasis, but conventional imaging limits characterization of the adrenal glands’ 3D structure. This study aimed to delineate the mouse adrenal glands’ 3D architecture and key component spatial relationships via advanced tissue-clearing imaging.</p> Methods <p>We used FDISCO (fluorescence-preserving 3D imaging of solvent-cleared organs) for whole-mouse adrenal imaging, complemented by 2D cortical and medullary marker co-staining and 3D density quantification.</p> Results <p>3D imaging revealed three key features of mouse adrenal glands. First, sympathetic nerves primarily surround adrenal blood vessels, forming a close spatial association with the vascular network. Second, the cortex and medulla were tightly interwoven. Third, we characterized the 3D vasculature of adrenocortical accessory nodules, which are devoid of medullary components and have significantly higher vascular density than the cortex.</p> Conclusions <p>We defined mouse adrenal 3D architecture, providing novel insights and highlighting FDISCO’s value for adrenal research.</p>

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High-Resolution 3D reconstruction and visualization of normal adrenal vasculature and sympathetic nerves in mice

  • 期刊论文

  • 发表日期：

    2026年2月16日

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  • Shuangshuang Zhu,
  • Junhao Fu,
  • Qinglin Liu,
  • Manman Du,
  • Linqiang Ma,
  • Jinbo Hu,
  • Chuan Peng,
  • Yunyan Liu,
  • Juan Yin,
  • Yong Xu,
  • Wei Huang,
  • Rufei Gao,
  • Qifu Li,
  • Shumin Yang,
  • Yunjie Xiong

摘要

Purpose

The vasculature and sympathetic nervous system are critical for adrenal homeostasis, but conventional imaging limits characterization of the adrenal glands’ 3D structure. This study aimed to delineate the mouse adrenal glands’ 3D architecture and key component spatial relationships via advanced tissue-clearing imaging.

Methods

We used FDISCO (fluorescence-preserving 3D imaging of solvent-cleared organs) for whole-mouse adrenal imaging, complemented by 2D cortical and medullary marker co-staining and 3D density quantification.

Results

3D imaging revealed three key features of mouse adrenal glands. First, sympathetic nerves primarily surround adrenal blood vessels, forming a close spatial association with the vascular network. Second, the cortex and medulla were tightly interwoven. Third, we characterized the 3D vasculature of adrenocortical accessory nodules, which are devoid of medullary components and have significantly higher vascular density than the cortex.

Conclusions

We defined mouse adrenal 3D architecture, providing novel insights and highlighting FDISCO’s value for adrenal research.