<p>Rheumatoid arthritis (RA) is the most prevalent form of inflammatory arthritis globally. It is believed to arise from a combination of genetic predisposition and environmental factors. Several investigations have emphasized the critical role of long non-coding RNAs (lncRNAs) in both the initiation and progression of RA. This study focuses on the potential link between the rs217727 and rs2839698 variants of the <i>H19</i> and RA susceptibility. The study involved 152 patients with clinically confirmed RA and 150 age- and gender-matched controls. A kit was utilized to extract the DNA of all the samples. After preparing the samples, DNA genotyping was done by using the tetra-primer ARMS-PCR method. The multivariate logistic regression analysis indicated that individuals with the CT genotype of rs217727 have a 2.28 times greater risk of developing RA than those with the TT genotype (<i>P</i> = 0.016). Furthermore, a significant 1.96-fold increase in RA risk was observed in patients with the CT genotype compared with those with the CC + TT genotype (<i>P</i> = 0.003). In addition, the rs2839698 variant of the <i>H19</i> was found to elevate the risk of RA within the co-dominant model (OR = 2.71, 95% CI = 1.51–4.86, <i>P</i> = 0.0006 for CT genotype), dominant model (OR = 1.87, 95% CI = 1.11–3.14, <i>P</i> = 0.016), and over-dominant model (OR = 2.37, 95% CI = 1.47–3.83, <i>P</i> = 0.0003). The significant association remains unchanged after applying the Bonferroni correction for multiple comparisons (adjusted <i>p</i> &lt; 0.025). The CT haplotype was initially associated with a reduced risk of developing RA (OR = 0.700, <i>p</i> = 0.047); however, this association did not remain statistically significant after applying the Bonferroni correction for multiple comparisons (adjusted <i>p</i> &lt; 0.0125). This research identified a link between genetic variations in <i>H19</i> and an increased likelihood of developing RA within the Iranian population.</p>