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<p>This study investigates the anticancer effects and the underlying molecular mechanisms of Mimulone on human hepatoma Hep3B cells. Mimulone was found to inhibit cell proliferation and induce apoptosis in Hep3B cells, as demonstrated by annexin V-fluorescein isothiocyanate staining and flow cytometry analyses. Western blot analysis revealed that Mimulone treatment decreased the levels of procaspase-3 and PARP, while increasing the levels of cleaved caspase-3, −8, PARP, Fas, FasL, and FADD. These results suggest that Mimulone induces apoptotic cell death through the activation of the death receptor-mediated (extrinsic) pathway. Furthermore, Mimulone inactivated the extracellular signal-regulated kinase (ERK) and Akt signaling pathways, further enhancing its pro-apoptotic effects. These findings indicate that Mimulone has potential as a therapeutic agent targeting apoptosis and survival signaling pathways in hepatocellular carcinoma cells.</p>

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Mimulone-induced Apoptosis through the Extrinsic Pathway and ERK Inactivation in Human Hepatoma Hep3B Cells

  • 期刊论文

  • 发表日期：

    2025年7月30日

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  • Hyunju Kim

摘要

This study investigates the anticancer effects and the underlying molecular mechanisms of Mimulone on human hepatoma Hep3B cells. Mimulone was found to inhibit cell proliferation and induce apoptosis in Hep3B cells, as demonstrated by annexin V-fluorescein isothiocyanate staining and flow cytometry analyses. Western blot analysis revealed that Mimulone treatment decreased the levels of procaspase-3 and PARP, while increasing the levels of cleaved caspase-3, −8, PARP, Fas, FasL, and FADD. These results suggest that Mimulone induces apoptotic cell death through the activation of the death receptor-mediated (extrinsic) pathway. Furthermore, Mimulone inactivated the extracellular signal-regulated kinase (ERK) and Akt signaling pathways, further enhancing its pro-apoptotic effects. These findings indicate that Mimulone has potential as a therapeutic agent targeting apoptosis and survival signaling pathways in hepatocellular carcinoma cells.