<p>Extended exposure to heavy metals, even at low concentrations, can negatively affect human semen quality and may contribute to male infertility. Therefore, this study was designed to provide firsthand information on the prevalence of elements in human seminal plasma and identify potential associations between levels of elements and semen quality parameters (motility, count, and morphology) of the Jordanian population. The concentrations of 17 elements in 97 seminal plasma samples were determined simultaneously using inductively coupled plasma mass spectrometry (ICP-MS). Among all analyzed elements, ⁴⁸Ca and ⁵²Cr were found to be the most abundant in the seminal fluid. No significant associations were observed between the concentration of essential elements and sperm morphology or count. However, elevated concentrations of ¹¹⁵In and ¹¹⁴Cd were strongly associated with reduced sperm motility and abnormal sperm morphology, indicating their potential reproductive toxicity. Moreover, significant associations were observed between low sperm motility and higher concentrations of ⁵¹V and ⁷⁵As. Interestingly, semen samples with normal parameters exhibited higher levels of ⁶⁹Ga, whereas samples with reduced motility were associated with elevated levels of essential elements including ⁵⁵Mn, ⁵²Cr, and ⁴⁸Ca. The validation parameters showed good reliability for the ICP-MS method, with good linearity (R² ˃ 0.997), and relative standard deviations (%RSD) below 5% for all target elements. This study examined several elements that have not been previously reported in the literature regarding seminal fluid, thereby providing new insights and contributing novel data to the existing body of scientific knowledge.</p>