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<p>The biosynthesized silver nanoparticles silver nanoparticles (Ag NPs) have considerable interest and attraction in recent years due to their ecofriendly, profitable and eco-accommodating characteristics. The purpose of this study was to biosynthesize the Ag NPs produced from <i>Rhazya stricta (</i>R. stricta) leaf extract to assess their antioxidant activity. The existence of phytochemicals including phlorotannin, terpenoids, glycosides, flavonoids, steroids, and, saponins are considered to reduce the silver ions into their metal form. R. stricta leaves extract was employed as a stabilizing and reducing agent. One major advantage of environmentally friendly methods of NPs over traditional physical and chemical processes is the absence of dangerous substances, high temperatures, pressure, and energy consumption. The morphological, optical, and chemical properties of the prepared Ag NPs were investigated using a variety of imaging and analytical techniques, included Energy dispersive X Rays spectroscopy (EDX), Scanning Electron Microscopy (SEM), ultraviolet visible (UV-Visible) and Fourier Transform Infrared (FTIR) Spectroscopy. The color formation served as visual confirmation that Ag nanoparticles were successfully synthesized. The UV-vis absorption peak appearing at 407&#xa0;nm confirmed the metal silver nature of as prepared NPs. SEM and EDX analysis confirmed that synthesized NPs were of almost of spherical nature with size ranging from 40 to 88&#xa0;nm. The FTIR spectrum signifies of presence of the diverted functional groups within biomolecules that encapsulate the Ag NPs. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was performed to verify Rs-leaves extract and Ag NP’s antioxidant efficacy. We demonstrated an excellent free radical scavenging ability of R. stricta-leaves extract and R. stricta-leaves mediated Ag NPs against antioxidant activity, and the IC<sub>50</sub> values were determined to be at 0.1&#xa0;mg/mL.</p>

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Biogenic Synthesis of Silver Nanoparticles from Rhazya Stricta Leaf Extract: Characterization and Free Radical Scavenging Activity

  • 期刊论文

  • 发表日期：

    2026年3月21日

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  • Hafiz Muhammad Sheraz,
  • Asif Ameen,
  • Abhinav Kumar,
  • Muhammad Zahid Shafiq,
  • Dhouha Choukaier,
  • Adel Noubigh,
  • Wajeehah Shahid,
  • Ankit Dilipkumar Oza

摘要

The biosynthesized silver nanoparticles silver nanoparticles (Ag NPs) have considerable interest and attraction in recent years due to their ecofriendly, profitable and eco-accommodating characteristics. The purpose of this study was to biosynthesize the Ag NPs produced from Rhazya stricta (R. stricta) leaf extract to assess their antioxidant activity. The existence of phytochemicals including phlorotannin, terpenoids, glycosides, flavonoids, steroids, and, saponins are considered to reduce the silver ions into their metal form. R. stricta leaves extract was employed as a stabilizing and reducing agent. One major advantage of environmentally friendly methods of NPs over traditional physical and chemical processes is the absence of dangerous substances, high temperatures, pressure, and energy consumption. The morphological, optical, and chemical properties of the prepared Ag NPs were investigated using a variety of imaging and analytical techniques, included Energy dispersive X Rays spectroscopy (EDX), Scanning Electron Microscopy (SEM), ultraviolet visible (UV-Visible) and Fourier Transform Infrared (FTIR) Spectroscopy. The color formation served as visual confirmation that Ag nanoparticles were successfully synthesized. The UV-vis absorption peak appearing at 407 nm confirmed the metal silver nature of as prepared NPs. SEM and EDX analysis confirmed that synthesized NPs were of almost of spherical nature with size ranging from 40 to 88 nm. The FTIR spectrum signifies of presence of the diverted functional groups within biomolecules that encapsulate the Ag NPs. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was performed to verify Rs-leaves extract and Ag NP’s antioxidant efficacy. We demonstrated an excellent free radical scavenging ability of R. stricta-leaves extract and R. stricta-leaves mediated Ag NPs against antioxidant activity, and the IC50 values were determined to be at 0.1 mg/mL.