Scholarlink

<p>In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated and identified as <i>Sphingobium yanoikuyae</i> SS02 using 16&#xa0;S rRNA gene sequencing. Optimization of the culture medium through a one-factor-at-a-time (OFAT) approach enhanced EPS yield to 6.5 ± 0.2&#xa0;g/L under mesophilic conditions. The purified EPS was structurally and functionally characterized using UV-Visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), and Thermogravimetric Analysis (TGA). Spectroscopic analyses confirmed a glucose-rich heteropolysaccharide backbone with diverse functional groups, exhibiting moderate crystallinity and thermal stability, with major decomposition occurring between 150&#xa0;°C and 330&#xa0;°C. Biological evaluation revealed that the EPS possessed concentration-dependent antioxidant activity, with an IC50 of 0.635&#xa0;mg/mL in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and 92.5% and 78.3% inhibition for ascorbic acid and EPS, respectively, in the phosphomolybdenum assay. The EPS also exhibited notable anti-inflammatory activity with 72.57% inhibition at 800&#xa0;µg/ml, and broad-spectrum antibacterial activity against <i>Staphylococcus aureus</i>, <i>Klebsiella pneumoniae</i>, <i>Bacillus subtilis</i>, and <i>Escherichia coli</i>, with the highest sensitivity observed in <i>S. aureus</i>. These findings demonstrate the multifunctional bioactivity and biocompatibility of <i>S. yanoikuyae</i> SS02-derived EPS, suggesting its potential as a natural therapeutic agent for pharmaceutical and biomedical applications.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Bioprospecting of Sphingobium yanoikuyae SS02 for Exopolysaccharide Production: Optimization, Characterization, and Therapeutic Profiling

  • 期刊论文

  • 发表日期：

    2026年3月14日

查看全文
  • Vishruthi Sundar Kumar,
  • Preethi Kathirvel

摘要

In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated and identified as Sphingobium yanoikuyae SS02 using 16 S rRNA gene sequencing. Optimization of the culture medium through a one-factor-at-a-time (OFAT) approach enhanced EPS yield to 6.5 ± 0.2 g/L under mesophilic conditions. The purified EPS was structurally and functionally characterized using UV-Visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Nuclear Magnetic Resonance (NMR), and Thermogravimetric Analysis (TGA). Spectroscopic analyses confirmed a glucose-rich heteropolysaccharide backbone with diverse functional groups, exhibiting moderate crystallinity and thermal stability, with major decomposition occurring between 150 °C and 330 °C. Biological evaluation revealed that the EPS possessed concentration-dependent antioxidant activity, with an IC50 of 0.635 mg/mL in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and 92.5% and 78.3% inhibition for ascorbic acid and EPS, respectively, in the phosphomolybdenum assay. The EPS also exhibited notable anti-inflammatory activity with 72.57% inhibition at 800 µg/ml, and broad-spectrum antibacterial activity against Staphylococcus aureus, Klebsiella pneumoniae, Bacillus subtilis, and Escherichia coli, with the highest sensitivity observed in S. aureus. These findings demonstrate the multifunctional bioactivity and biocompatibility of S. yanoikuyae SS02-derived EPS, suggesting its potential as a natural therapeutic agent for pharmaceutical and biomedical applications.

Graphical Abstract