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<p>The production and consumption of plant-based foods are increasing due to health, ethical, and environmental concerns. However, plants do not synthesize vitamin B<sub>12</sub>, and the fortification of plant-based foods with this vitamin is highly recommended to prevent its deficiency. This work aimed to optimize vitamin B<sub>12</sub> production by <i>Propionibacterium freudenreichii</i> subsp. <i>shermanii</i> ATCC 13673 growing on stirred tank bioreactors using the liquid acid protein residue of soybean, an agroindustrial waste, as medium culture. The influence of medium supplementation, presaccharification of sugars, and aeration strategies was investigated. The pH and temperature controls were optimized by applying the design of experiments (doe) approach, based on a central composite design. Vitamin B<sub>12</sub> production more than tripled (from ~ 1.5&#xa0;mg • L<sup>−1</sup> to 5&#xa0;mg • L<sup>−1</sup>) after the optimization process. Cultures of <i>P. freudenreichii</i> produced high concentrations of biomass (&gt; 6&#xa0;g • L<sup>−1</sup>), increasing by threefold the specific biomass yield of vitamin B<sub>12</sub> (&gt; 0.8&#xa0;mg • g<sup>−1</sup> dry cell). This bioprocess, based on the use of cheap and readily available soybean agroindustrial waste as microbial medium, and using a GRAS microorganism, could become an efficient alternative source of vitamin B<sub>12</sub> production to fortify plant-based products.</p>

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Production of Vitamin B12 by Propionibacterium freudenreichii subsp. shermanii ATCC 13673 on a Soybean Agroindustrial Waste-Based Medium: Bioprocess Optimization in STR Bioreactors

  • 期刊论文

  • 发表日期：

    2025年11月15日

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  • Dener Acosta de Assis,
  • Marco Antônio Záchia Ayub

摘要

The production and consumption of plant-based foods are increasing due to health, ethical, and environmental concerns. However, plants do not synthesize vitamin B12, and the fortification of plant-based foods with this vitamin is highly recommended to prevent its deficiency. This work aimed to optimize vitamin B12 production by Propionibacterium freudenreichii subsp. shermanii ATCC 13673 growing on stirred tank bioreactors using the liquid acid protein residue of soybean, an agroindustrial waste, as medium culture. The influence of medium supplementation, presaccharification of sugars, and aeration strategies was investigated. The pH and temperature controls were optimized by applying the design of experiments (doe) approach, based on a central composite design. Vitamin B12 production more than tripled (from ~ 1.5 mg • L−1 to 5 mg • L−1) after the optimization process. Cultures of P. freudenreichii produced high concentrations of biomass (> 6 g • L−1), increasing by threefold the specific biomass yield of vitamin B12 (> 0.8 mg • g−1 dry cell). This bioprocess, based on the use of cheap and readily available soybean agroindustrial waste as microbial medium, and using a GRAS microorganism, could become an efficient alternative source of vitamin B12 production to fortify plant-based products.