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<p>Fluoride in drinking water poses health risks when exceeding WHO-recommended limits, necessitating accurate detection methods. This study developed a highly selective fluoride riboswitch (FRS)-based whole-cell biosensor using an <i>E. coli</i> mutant with a recombinant plasmid carrying the FRS fused to the <i>lacZ</i> reporter gene. Fluoride binding to the FRS aptamer triggers β-galactosidase expression, enabling fluoride quantification by a colorimetric assay. Using a calibration curve (<i>R</i><sup>2</sup> &gt; 0.9), fluoride concentrations in Sri Lankan water samples were determined (0.15–0.90&#xa0;ppm). Minimal interference from common ions (Cl⁻, OH⁻, Na⁺, K⁺, Ca<sup>2</sup>⁺, CO₃<sup>2</sup>⁻, HCO₃⁻, and NO₃⁻) confirmed the biosensor’s narrow specificity and high selectivity towards fluoride.</p> Graphical Abstract <p></p>

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Simple and Cost-effective Fluoride Riboswitch-Based Whole-Cell Biosensor for the Determination of Fluoride in Drinking Water

  • 期刊论文

  • 发表日期：

    2025年5月24日

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  • Madushani R. Ariyarathna,
  • Jayani P. Nissanka,
  • Kasun Methlal,
  • Kasun D. Abeyrathne,
  • Madhusha Satharasinghe,
  • P. Banushan,
  • Dilhara Manawadu,
  • Gayan Mirihana Arachchilage,
  • Gayathri N. Silva

摘要

Fluoride in drinking water poses health risks when exceeding WHO-recommended limits, necessitating accurate detection methods. This study developed a highly selective fluoride riboswitch (FRS)-based whole-cell biosensor using an E. coli mutant with a recombinant plasmid carrying the FRS fused to the lacZ reporter gene. Fluoride binding to the FRS aptamer triggers β-galactosidase expression, enabling fluoride quantification by a colorimetric assay. Using a calibration curve (R2 > 0.9), fluoride concentrations in Sri Lankan water samples were determined (0.15–0.90 ppm). Minimal interference from common ions (Cl⁻, OH⁻, Na⁺, K⁺, Ca2⁺, CO₃2⁻, HCO₃⁻, and NO₃⁻) confirmed the biosensor’s narrow specificity and high selectivity towards fluoride.

Graphical Abstract