Scholarlink

<p>This study aimed to develop probiotic microcapsules with high viability of dual probiotics, lactic acid bacteria (LAB), and yeast, using goat milk powder. Freeze-dried probiotic microcapsules (FDPM) exhibited higher encapsulation efficiency and production yield than spray-dried microcapsules. Composite cryoprotectants, i.e., gum Arabic, inulin, and disodium hydrogen phosphate (Na<sub>2</sub>HPO<sub>4</sub>) were optimized through response surface methodology (RSM). The optimal formulations to maximize both encapsulation efficiency and production yield were 9.42% gum Arabic, 1.03% inulin, and 0.42% Na<sub>2</sub>HPO<sub>4</sub>, giving the maximum encapsulation efficiency of LAB at 86.6 ± 0.4% and yeast at 94.6 ± 1.0%. <i>In vitro</i> gastrointestinal tests indicated that both LAB and yeast probiotics under the protection of the FDPM formula could tolerate gastrointestinal fluids with high survival rates &gt; 95% for LAB and &gt; 85% for yeast, indicating their high potential to reach the intestine smoothly. The storage stability tests showed that the viable counts of probiotics in FDPM remained above 8 log CFU/g after 60&#xa0;days of storage at 4&#xa0;°C and – 18&#xa0;°C, exceeding the minimum level generally required for probiotic efficacy. Furthermore, FDPM was applied in an effervescent tablet. The results showed that the effervescent tablet fortified with 31% (w/w) FDPM (PE-1) exhibited the highest survival rates of 97.8% for LAB and 93.1% for yeast after tableting and a disintegration time &lt; 5&#xa0;min. These results validate that the freeze-drying technique coupled with optimized composite cryoprotectants could protect probiotics during delivery through the gastrointestinal tract and storage and can also be applied in effervescent tablets, providing a promising strategy for developing convenient and shelf-stable probiotic supplements.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Optimization of Composite Cryoprotectants to Improve Viability of Freeze-Dried Probiotic Microcapsules of Lactic Acid Bacteria and Yeast and Application in Effervescent Tablets

  • 期刊论文

  • 发表日期：

    2026年7月3日

查看全文
  • Asma Billateh,
  • Benjamas Cheirsilp,
  • Wasana Suyotha

摘要

This study aimed to develop probiotic microcapsules with high viability of dual probiotics, lactic acid bacteria (LAB), and yeast, using goat milk powder. Freeze-dried probiotic microcapsules (FDPM) exhibited higher encapsulation efficiency and production yield than spray-dried microcapsules. Composite cryoprotectants, i.e., gum Arabic, inulin, and disodium hydrogen phosphate (Na2HPO4) were optimized through response surface methodology (RSM). The optimal formulations to maximize both encapsulation efficiency and production yield were 9.42% gum Arabic, 1.03% inulin, and 0.42% Na2HPO4, giving the maximum encapsulation efficiency of LAB at 86.6 ± 0.4% and yeast at 94.6 ± 1.0%. In vitro gastrointestinal tests indicated that both LAB and yeast probiotics under the protection of the FDPM formula could tolerate gastrointestinal fluids with high survival rates > 95% for LAB and > 85% for yeast, indicating their high potential to reach the intestine smoothly. The storage stability tests showed that the viable counts of probiotics in FDPM remained above 8 log CFU/g after 60 days of storage at 4 °C and – 18 °C, exceeding the minimum level generally required for probiotic efficacy. Furthermore, FDPM was applied in an effervescent tablet. The results showed that the effervescent tablet fortified with 31% (w/w) FDPM (PE-1) exhibited the highest survival rates of 97.8% for LAB and 93.1% for yeast after tableting and a disintegration time < 5 min. These results validate that the freeze-drying technique coupled with optimized composite cryoprotectants could protect probiotics during delivery through the gastrointestinal tract and storage and can also be applied in effervescent tablets, providing a promising strategy for developing convenient and shelf-stable probiotic supplements.

Graphical Abstract