Scholarlink

<p>Plant proteins often suffer from structural limitations and poor interfacial performance, leading to low delivery efficiency of bioactive compounds. To overcome these challenges, this study developed a synergistic modification strategy combining alkaline pH-shifting and ultrasonication. By controlling the protein concentration (1, 2, 4%, w/v) and water–oil ratio (7:3, 6:4, 5:5, v/v) of soybean lipophilic protein (SLP), we aimed to achieve efficient co-delivery of vitamin E (VE) and quercetin (Que). The results demonstrated that the synergistic strategy effectively disrupted the hydrophobic aggregation of SLP, significantly reducing particle size and increasing the absolute zeta potential (<i>P</i> &lt; 0.05). After modification, the interfacial activity of SLP was enhanced, promoting rapid protein adsorption at the oil–water interface and the formation of a dense interfacial film. The maximum interfacial protein adsorption reached 77.49%, and the flocculation index decreased to a minimum of 8.67%. Consequently, the encapsulation efficiencies of VE and Que were substantially increased from 44.74% and 33.00% to 73.82% and 72.27%, respectively (<i>P</i> &lt; 0.05). Stability analysis revealed that protein concentration and water–oil ratio enhanced the physical stability of the emulsion by regulating interfacial film strength and emulsion viscoelasticity. In vitro digestion further demonstrated that the modified SLP emulsion achieved maximum bioavailability of VE and Que, reaching 42.83% and 38.60%, respectively, at 2% protein concentration and 6:4 water–oil ratio. These findings provide important technical support for the development of plant protein-based co-delivery systems for bioactive compounds, and also offer practical insights for the efficient utilization of active ingredients in functional foods.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Synergistic Effects of pH-Shifting and Ultrasonication on Interfacial Engineering of Soybean Lipophilic Protein: Toward Enhanced Emulsion Stability and Efficient Vitamin Co-delivery

  • 期刊论文

  • 发表日期：

    2026年4月13日

查看全文
  • Xumei Feng,
  • Lijia Li,
  • Yue Liu,
  • Yang Li,
  • Fei Teng

摘要

Plant proteins often suffer from structural limitations and poor interfacial performance, leading to low delivery efficiency of bioactive compounds. To overcome these challenges, this study developed a synergistic modification strategy combining alkaline pH-shifting and ultrasonication. By controlling the protein concentration (1, 2, 4%, w/v) and water–oil ratio (7:3, 6:4, 5:5, v/v) of soybean lipophilic protein (SLP), we aimed to achieve efficient co-delivery of vitamin E (VE) and quercetin (Que). The results demonstrated that the synergistic strategy effectively disrupted the hydrophobic aggregation of SLP, significantly reducing particle size and increasing the absolute zeta potential (P < 0.05). After modification, the interfacial activity of SLP was enhanced, promoting rapid protein adsorption at the oil–water interface and the formation of a dense interfacial film. The maximum interfacial protein adsorption reached 77.49%, and the flocculation index decreased to a minimum of 8.67%. Consequently, the encapsulation efficiencies of VE and Que were substantially increased from 44.74% and 33.00% to 73.82% and 72.27%, respectively (P < 0.05). Stability analysis revealed that protein concentration and water–oil ratio enhanced the physical stability of the emulsion by regulating interfacial film strength and emulsion viscoelasticity. In vitro digestion further demonstrated that the modified SLP emulsion achieved maximum bioavailability of VE and Que, reaching 42.83% and 38.60%, respectively, at 2% protein concentration and 6:4 water–oil ratio. These findings provide important technical support for the development of plant protein-based co-delivery systems for bioactive compounds, and also offer practical insights for the efficient utilization of active ingredients in functional foods.

Graphical Abstract