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<p>The identification of genetically modified (GM) food is very important in the cross-border circulation of foods due to different regulatory policies between different countries. Current detection methods mainly rely on nucleic acid amplification techniques, lacking simple, stable, and low-cost approaches. In this work, we have proposed a novel strategy for amplification-free monitoring GM food (taking GM corn MON810 as an example) based on polyelectrolyte-induced self-assembly between the perylene derivative (PBTMA) and single-stranded DNA (MP). A supramolecular complex of PBTMA/MP was formed driven by the non-covalent interaction, resulting in fluorescence quenching. In the presence of MON810 target DNA, a double helix structure was formed between MP and the target DNA, weakening the interaction strength of PBTMA/MP and restoring fluorescence, thereby enabling detection of MON810. The detection linear range was 5–100&#xa0;nM, with a limit of detection (LOD) of 1.17&#xa0;nM. This strategy successfully distinguished and detected MON810 in actual samples and is expected to be extended to all GM food. To our knowledge, this work is the first to report the use of a polyelectrolyte-induced self-assembly strategy for enzyme-free and amplification-free detection of GM food.</p> Graphical Abstract <p></p>

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Enzyme-Free and Amplification-Free Detection of Genetically Modified Food Based on Polyelectrolyte-Induced Self-assembly of Water-Soluble Fluorescent Probe

  • 期刊论文

  • 发表日期：

    2026年2月17日

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  • Shuai Zhou,
  • Yanping Jiang,
  • Ge Shi,
  • Tianyue Liu,
  • Jingsheng Tian,
  • Yao Shen,
  • Kunlun Huang,
  • Jie Liu,
  • Zhiyi Yao

摘要

The identification of genetically modified (GM) food is very important in the cross-border circulation of foods due to different regulatory policies between different countries. Current detection methods mainly rely on nucleic acid amplification techniques, lacking simple, stable, and low-cost approaches. In this work, we have proposed a novel strategy for amplification-free monitoring GM food (taking GM corn MON810 as an example) based on polyelectrolyte-induced self-assembly between the perylene derivative (PBTMA) and single-stranded DNA (MP). A supramolecular complex of PBTMA/MP was formed driven by the non-covalent interaction, resulting in fluorescence quenching. In the presence of MON810 target DNA, a double helix structure was formed between MP and the target DNA, weakening the interaction strength of PBTMA/MP and restoring fluorescence, thereby enabling detection of MON810. The detection linear range was 5–100 nM, with a limit of detection (LOD) of 1.17 nM. This strategy successfully distinguished and detected MON810 in actual samples and is expected to be extended to all GM food. To our knowledge, this work is the first to report the use of a polyelectrolyte-induced self-assembly strategy for enzyme-free and amplification-free detection of GM food.

Graphical Abstract