Harnessing Saltatory Rolling Circle Amplification and Lateral Flow Strip (SRCA-LFS) for Rapid Onsite Detection of Listeria monocytogenes in Meat Products
摘要
Listeria monocytogenes, as one of the most virulent and high-risk foodborne pathogens, is a well-documented causative agent of listeriosis that jeopardizes human health. This bacterium exhibits widespread distribution across diverse food categories, with particularly high prevalence in meat products. Consequently, the development of rapid and sensitive detection methods for L. monocytogenes is critically important for ensuring public health and food safety. Herein, we developed an innovative detection approach by synergistically integrating the high sensitivity of saltatory rolling circle amplification (SRCA) with the rapid visual interpretation capability of lateral flow strips (LFS). This SRCA-LFS method enables efficient onsite detection of L. monocytogenes in meat products, significantly shortening the detection time and enhancing field applicability. Specifically, the proposed method can detect L. monocytogenes in meat products within 40 min. The SRCA-LFS assay demonstrated a sensitivity of 2.94 × 100 fg/μL and a limit of detection of 3.46 × 100 CFU/mL, which are comparable to those of real-time fluorescence SRCA and 100-fold superior to PCR-LFS. The relative sensitivity, specificity, and accuracy of this method were 100.00%, 97.62%, and 98.00%, respectively. These results highlight that the SRCA-LFS method, characterized by operational simplicity, high efficiency, and user-friendly design, holds excellent potential for onsite detection of foodborne pathogens.