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<p>This study investigates the effect of ozone elicitation on the antioxidant status of radish sprouts. One-day-old sprouts were ozonated at a concentration of 50&#xa0;ppm for 1, 15, 30, and 60&#xa0;min. After 6&#xa0;days of germination, the biomass was analyzed for antioxidant status, including levels of polyphenols, glutathione, and ascorbate, as well as oxidative stress markers and indicators of energy metabolism. The results demonstrated that the impact of ozone treatment was significantly influenced by the duration of the process. The use of 30-min elicitation resulted in a significant increase in polyphenol content by 14% and ascorbate by 12% compared to the control. However, 60-min exposure to ozone induced glutathione production by 25%. These increases in phytochemicals were accompanied by enhanced expression of proteins involved in the biosynthesis of the corresponding substances as well as elevated levels of ABA and presumed ethylene. It was also noted that ozonated sprouts are characterized by higher expression and activity of antioxidant enzymes as well as increased TBARS levels, thus proving the production of ROS during ozonation and their role in intracellular signaling. In addition, ozone treatment improved energy metabolism, as evidenced by increased expression of respiratory proteins and higher ATP content.</p>

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Ozone Treatment Enhances Antioxidant Status and Energy Metabolism in Radish (Raphanus sativus) Sprouts

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月15日

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  • Tomasz Piechowiak,
  • Ireneusz Kapusta,
  • Małgorzata Rak,
  • Maciej Balawejder

摘要

This study investigates the effect of ozone elicitation on the antioxidant status of radish sprouts. One-day-old sprouts were ozonated at a concentration of 50 ppm for 1, 15, 30, and 60 min. After 6 days of germination, the biomass was analyzed for antioxidant status, including levels of polyphenols, glutathione, and ascorbate, as well as oxidative stress markers and indicators of energy metabolism. The results demonstrated that the impact of ozone treatment was significantly influenced by the duration of the process. The use of 30-min elicitation resulted in a significant increase in polyphenol content by 14% and ascorbate by 12% compared to the control. However, 60-min exposure to ozone induced glutathione production by 25%. These increases in phytochemicals were accompanied by enhanced expression of proteins involved in the biosynthesis of the corresponding substances as well as elevated levels of ABA and presumed ethylene. It was also noted that ozonated sprouts are characterized by higher expression and activity of antioxidant enzymes as well as increased TBARS levels, thus proving the production of ROS during ozonation and their role in intracellular signaling. In addition, ozone treatment improved energy metabolism, as evidenced by increased expression of respiratory proteins and higher ATP content.