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<p>Nisin is a natural antimicrobial peptide widely used as a food preservative. The demand for high-purity nisin is also increasing because of its potential application in antimicrobial food packaging to extend shelf life and enhance food safety. This study introduced an innovative purification process to produce high-purity nisin. The desorption conditions for cell-bound nisin and the parameters for ion-exchange chromatography were subsequently optimized. A novel salt-free hydrochloric acid–ethanol solution was employed as the eluent, yielding nisin with a specific activity of 80,216.43 international units (IU)/mg protein. The freeze-dried product obtained from the ion-exchange chromatography elution represented high-purity nisin Z, with a 36,372.66 IU/mg titer. Stability tests confirmed the reproducibility of the process, achieving a high-purity nisin yield of 94.42%. Both liquid and solid high-purity nisin were characterized using high-performance liquid chromatography and liquid chromatography-mass spectrometry. This streamlined process substantially enhanced the purity and yield of nisin.</p>

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Innovative Process for High-Purity Nisin Z Recovery Using Cell-Bound Adsorption and Ion-Exchange Chromatography

  • 期刊论文

  • 发表日期：

    2025年12月16日

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  • Hao Dong,
  • Shijie Zhang,
  • Aimin He,
  • Liang Wang,
  • Hongjian Zhang,
  • Jianhua Zhang,
  • Xusheng Chen

摘要

Nisin is a natural antimicrobial peptide widely used as a food preservative. The demand for high-purity nisin is also increasing because of its potential application in antimicrobial food packaging to extend shelf life and enhance food safety. This study introduced an innovative purification process to produce high-purity nisin. The desorption conditions for cell-bound nisin and the parameters for ion-exchange chromatography were subsequently optimized. A novel salt-free hydrochloric acid–ethanol solution was employed as the eluent, yielding nisin with a specific activity of 80,216.43 international units (IU)/mg protein. The freeze-dried product obtained from the ion-exchange chromatography elution represented high-purity nisin Z, with a 36,372.66 IU/mg titer. Stability tests confirmed the reproducibility of the process, achieving a high-purity nisin yield of 94.42%. Both liquid and solid high-purity nisin were characterized using high-performance liquid chromatography and liquid chromatography-mass spectrometry. This streamlined process substantially enhanced the purity and yield of nisin.