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<p>Induced electric field (IEF), as a novel electrotechnology, has a potential inactivation effect against pathogenic microorganisms. However, little is known about the antibacterial mechanism during the IEF treatment. In this study, the germicidal efficacy and inactivation mechanism of IEF against <i>E. coli</i> were investigated. After IEF treatment, the number of <i>E. coli</i> in selected three liquid foods, namely milk, orange juice, and normal saline, was reduced by about 7–7.5 Log<sub>10</sub> CFU/mL; the maximum proportion of sublethal injury was 86.83%, 69.14%, and 76.47%, respectively. Moreover, microscopic analysis, including SEM, TEM, and CLSM, showed that IEF treatment damaged the membrane morphology, integrity, and intracellular substructure of <i>E. coli</i>. The conspicuous dissipation of membrane potential and decrease in esterase activity after IEF treatment were also observed by fluorimetry. The SDS-PAGE and CD spectra analysis indicated that IEF treatment altered the secondary structure of intracellular proteins but showed no significant effect on the primary structure.</p>

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Inactivation Effects on Escherichia coli in Selected Liquid Food Models by Induced Electric Field: Germicidal Efficacy and Putative Mechanism

  • 期刊论文

  • 发表日期：

    2025年6月10日

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  • Lingtao Zhang,
  • Yamei Jin,
  • Liping Xue,
  • Huang Zhang,
  • Na Yang,
  • Xueming Xu

摘要

Induced electric field (IEF), as a novel electrotechnology, has a potential inactivation effect against pathogenic microorganisms. However, little is known about the antibacterial mechanism during the IEF treatment. In this study, the germicidal efficacy and inactivation mechanism of IEF against E. coli were investigated. After IEF treatment, the number of E. coli in selected three liquid foods, namely milk, orange juice, and normal saline, was reduced by about 7–7.5 Log10 CFU/mL; the maximum proportion of sublethal injury was 86.83%, 69.14%, and 76.47%, respectively. Moreover, microscopic analysis, including SEM, TEM, and CLSM, showed that IEF treatment damaged the membrane morphology, integrity, and intracellular substructure of E. coli. The conspicuous dissipation of membrane potential and decrease in esterase activity after IEF treatment were also observed by fluorimetry. The SDS-PAGE and CD spectra analysis indicated that IEF treatment altered the secondary structure of intracellular proteins but showed no significant effect on the primary structure.