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<p>This study evaluated the effects of laser, ultrasound, and their combined treatments on the morphometric, phytochemical, techno-functional, and physicochemical properties of broccoli, radish, and kale seeds, sprouts, and microgreens. Treated seeds underwent controlled germination and sprouting, followed by analyses including germination rate, sprout/root length, fresh weight, total phenolic content (TPC), DPPH radical-scavenging activity, total flavonoid content (TFC), protein solubility, water/oil absorption capacity, emulsifying activity/stability, foaming capacity/stability, and color attributes. Combined laser and ultrasound treatments significantly enhanced germination and growth characteristics: germination rates increased by 13%, 11%, and 10% for kale, radish, and broccoli, respectively, with average sprout lengths increasing by 32.61%, 28.30%, and 14.04%. Phytochemical analysis revealed significant increases in TPC, DPPH activity, and TFC, especially in treated sprouts, highlighting the synergistic effects on antioxidant capacity. DPPH activity rose from 4.25 to 9.56&#xa0;μmol TE/g dry weight (d.w) for broccoli, 15.39 to 25.56&#xa0;μmol TE/g d.w for radish, and 17.15 to 27.01&#xa0;μmol TE/g d.w for kale. Techno-functional properties, including protein solubility and emulsification capacity, were notably enhanced, particularly with hybrid treatment. Sprouts demonstrated higher techno-functionality than microgreens in most parameters. Color analysis indicated significant increases in <i>L*</i> and <i>b*</i> values, with decreases in <i>a*</i> values, particularly in samples treated with the combined method. Overall, these findings underscored the potential of hybrid laser-ultrasound technology for enhancing the nutritional and functional quality of plant materials, paving the way for improved functional food products.</p>

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Hybrid Laser/Ultrasound Technology Enhances Morphometric, Phytochemical, and Techno-Functional Properties of Seeds, Sprouts, and Microgreens of Broccoli, Radish, and Kale

  • 期刊论文

  • 发表日期：

    2025年5月29日

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  • Gulcin Yildiz,
  • Mengyi Dong,
  • Hao Feng

摘要

This study evaluated the effects of laser, ultrasound, and their combined treatments on the morphometric, phytochemical, techno-functional, and physicochemical properties of broccoli, radish, and kale seeds, sprouts, and microgreens. Treated seeds underwent controlled germination and sprouting, followed by analyses including germination rate, sprout/root length, fresh weight, total phenolic content (TPC), DPPH radical-scavenging activity, total flavonoid content (TFC), protein solubility, water/oil absorption capacity, emulsifying activity/stability, foaming capacity/stability, and color attributes. Combined laser and ultrasound treatments significantly enhanced germination and growth characteristics: germination rates increased by 13%, 11%, and 10% for kale, radish, and broccoli, respectively, with average sprout lengths increasing by 32.61%, 28.30%, and 14.04%. Phytochemical analysis revealed significant increases in TPC, DPPH activity, and TFC, especially in treated sprouts, highlighting the synergistic effects on antioxidant capacity. DPPH activity rose from 4.25 to 9.56 μmol TE/g dry weight (d.w) for broccoli, 15.39 to 25.56 μmol TE/g d.w for radish, and 17.15 to 27.01 μmol TE/g d.w for kale. Techno-functional properties, including protein solubility and emulsification capacity, were notably enhanced, particularly with hybrid treatment. Sprouts demonstrated higher techno-functionality than microgreens in most parameters. Color analysis indicated significant increases in L* and b* values, with decreases in a* values, particularly in samples treated with the combined method. Overall, these findings underscored the potential of hybrid laser-ultrasound technology for enhancing the nutritional and functional quality of plant materials, paving the way for improved functional food products.