Purpose of Review <p>This review discusses current trends in the implementation and application of two emerging optical microscopy techniques—light-sheet microscopy (LSM) and light-field microscopy (LFM)—in cardiovascular research. It covers the principles and advantages of these imaging modalities, highlighting their current and potential applications that are new to researchers in the cardiovascular field.</p> Recent Findings <p>LSM and LFM have proven invaluable in studying cardiac development, regeneration, and aging in health and disease. Recent advances include investigations of structural features such as heart valves, trabecular networks, and aortic arch in murine models; dynamic processes like cardiac contraction, hemodynamics, remodeling, and neural activities in zebrafish models; and disease models including myocardial infarction using cardiac organoids. With their high-speed volumetric acquisition capabilities, these imaging methods are particularly suited to assessing cardiac structures and functions in three dimension and four dimensions during cardiac morphogenesis, as well as its aberrant processes.</p> Summary <p>Given the unique advantages of LSM and LFM, their respective utilizations uncover structural and functional insights, underscoring a hitherto untapped potential to facilitate fundamental cardiovascular research.</p>