Scholarlink

<p>Although the extreme environmental conditions found in saline-alkaline soils severely restrict microbial survival and activity, certain hardy bacteria have developed defense mechanisms to survive and even flourish in these environments. A useful paradigm for comprehending microbial adaptation to dual stress was explored by the <i>Arthrobacter rhombi</i> BFL-3, which was isolated from fermented compost conditions. The global gene expression alterations in A. <i>rhombi</i> BFL-3 under saline (HS), alkaline (HA), and combined saline-alkaline (SA) stress conditions were examined in this work using transcriptome analysis in comparison to a non-stressed control (CK). After high-throughput RNA sequencing, enrichment analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and differential gene expression were conducted. The findings showed that A. <i>rhombi</i> BFL-3 significantly upregulated genes linked to translation, ribosome biogenesis, and peptide biosynthesis under all stress conditions, with the most pronounced response under saline-alkaline (SA) stress and increasing protein synthesis was a crucial adaptive tactic. A comprehensive metabolic reprogramming and regulatory modifications to preserve cellular function and homeostasis under stress were also shown by KEGG analysis, which revealed enrichment in the metabolism of amino acids, carbohydrates, energy, membrane transport, and signal transduction pathways. The requirement to preserve cellular integrity was also brought to light by the abundance of genes linked to ribonucleoprotein complexes and structural molecular function. To sum up, <i>Arthrobacter rhombi</i> BFL-3 uses a complex transcriptional response that includes upregulating metabolic, biosynthetic, and stress-response pathways in order to withstand saline-alkaline stress. These discoveries deepen our knowledge of the mechanisms behind microbial resilience and present promising avenues for biotechnology and saline-alkaline ecosystem environmental management.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Transcriptomic insights into the adaptation of Arthrobacter rhombi BFL-3 to saline-alkaline stress conditions

  • 期刊论文

  • 发表日期：

    2025年8月12日

查看全文
  • Yuxin Lu,
  • Qian Xu,
  • Tao Qin,
  • Yani Bai,
  • Hong Yan,
  • Wenjuan Zhao,
  • Zhizhen Feng,
  • Puyang Feng

摘要

Although the extreme environmental conditions found in saline-alkaline soils severely restrict microbial survival and activity, certain hardy bacteria have developed defense mechanisms to survive and even flourish in these environments. A useful paradigm for comprehending microbial adaptation to dual stress was explored by the Arthrobacter rhombi BFL-3, which was isolated from fermented compost conditions. The global gene expression alterations in A. rhombi BFL-3 under saline (HS), alkaline (HA), and combined saline-alkaline (SA) stress conditions were examined in this work using transcriptome analysis in comparison to a non-stressed control (CK). After high-throughput RNA sequencing, enrichment analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and differential gene expression were conducted. The findings showed that A. rhombi BFL-3 significantly upregulated genes linked to translation, ribosome biogenesis, and peptide biosynthesis under all stress conditions, with the most pronounced response under saline-alkaline (SA) stress and increasing protein synthesis was a crucial adaptive tactic. A comprehensive metabolic reprogramming and regulatory modifications to preserve cellular function and homeostasis under stress were also shown by KEGG analysis, which revealed enrichment in the metabolism of amino acids, carbohydrates, energy, membrane transport, and signal transduction pathways. The requirement to preserve cellular integrity was also brought to light by the abundance of genes linked to ribonucleoprotein complexes and structural molecular function. To sum up, Arthrobacter rhombi BFL-3 uses a complex transcriptional response that includes upregulating metabolic, biosynthetic, and stress-response pathways in order to withstand saline-alkaline stress. These discoveries deepen our knowledge of the mechanisms behind microbial resilience and present promising avenues for biotechnology and saline-alkaline ecosystem environmental management.