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Background <p>A growing body of evidence suggests that microRNAs (miRNAs) are key regulatory factors in cervical cancer (CC). However, the role and mechanism of action of miR-3074-5p in CC remain unclear.</p> Objective <p> The objective of this research is to elucidate the function of miR-3074-5p in CC.</p> Methods <p> Tumor tissues were collected from 141 CC patients. RT-qPCR was used to detect miR-3074-5p expression. Kaplan–Meier survival curves analyzed the association between the miR-3074-5p and 5-year overall survival rates. Cox regression models evaluated the potential prognostic value of miR-3074-5p. Cell proliferation was assessed using the CCK-8 assay, while cell migration and invasion capabilities were evaluated via Transwell experiments. Dual-luciferase reporter gene assays (DLR) and RNA immunoprecipitation (RIP) experiments identified miR-3074-5p target genes, with Pearson correlation analysis evaluated their associations.</p> Results <p> miR-3074-5p was markedly increased in CC. Its overexpression was closely associated with poor patient prognosis. Inhibiting miR-3074-5p expression effectively attenuated the malignant behavior of CC cells. miR-3074-5p directly targets TGFBR2, with inverse expression patterns confirmed in clinical samples. Rescue experiments demonstrated that TGFBR2 knockdown partially counteracted the tumor-suppressive effects induced by miR-3074-5p inhibition.</p> Conclusion <p>Downregulation of miR-3074-5p suppresses the malignant phenotype of CC cells by targeting TGFBR2. This finding provides initial insights into the cellular behavior of CC.</p>

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Downregulation of miR-3074-5p slows cervical cancer progression by targeting TGFBR2 expression

  • 期刊论文

  • 发表日期：

    2026年7月8日

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  • Xiaoqing Zhu,
  • Juan Xiang,
  • Jing Bai,
  • Lulu Wang,
  • Qing Tang

摘要

Background

A growing body of evidence suggests that microRNAs (miRNAs) are key regulatory factors in cervical cancer (CC). However, the role and mechanism of action of miR-3074-5p in CC remain unclear.

Objective

The objective of this research is to elucidate the function of miR-3074-5p in CC.

Methods

Tumor tissues were collected from 141 CC patients. RT-qPCR was used to detect miR-3074-5p expression. Kaplan–Meier survival curves analyzed the association between the miR-3074-5p and 5-year overall survival rates. Cox regression models evaluated the potential prognostic value of miR-3074-5p. Cell proliferation was assessed using the CCK-8 assay, while cell migration and invasion capabilities were evaluated via Transwell experiments. Dual-luciferase reporter gene assays (DLR) and RNA immunoprecipitation (RIP) experiments identified miR-3074-5p target genes, with Pearson correlation analysis evaluated their associations.

Results

miR-3074-5p was markedly increased in CC. Its overexpression was closely associated with poor patient prognosis. Inhibiting miR-3074-5p expression effectively attenuated the malignant behavior of CC cells. miR-3074-5p directly targets TGFBR2, with inverse expression patterns confirmed in clinical samples. Rescue experiments demonstrated that TGFBR2 knockdown partially counteracted the tumor-suppressive effects induced by miR-3074-5p inhibition.

Conclusion

Downregulation of miR-3074-5p suppresses the malignant phenotype of CC cells by targeting TGFBR2. This finding provides initial insights into the cellular behavior of CC.