Background <p>Non-pharmacological distraction methods are alternatives that can help relieve pain and anxiety during blood collection in pediatric patients.</p> Aims <p>This study aimed to evaluate the effects of distraction methods used during blood collection on pain and anxiety in children aged 9–13. Wong-Baker FACES Pain Rating Scale (WB-FACES) and Visual Analog Scale (VAS) were used to assess pain and anxiety levels.</p> Methods <p>A randomized controlled experimental study involving 450 pediatric patients aged 9–13 who applied to the pediatric endocrinology unit of a university hospital. During the blood collection procedure, children were randomly divided into 3 groups: watching cartoons, cold application (Buzzy), and control. Age-appropriate pain scales were used to assess pain.</p> Results <p>The study included 450 children, 57.6% (<i>n</i> = 259) male and 42.4% (<i>n</i> = 191) female, with a mean age of 11.02 ± 1.45. One-third (<i>n</i> = 150) of the participants were shown cartoons, and one-third (<i>n</i> = 150) were given the cold application method. The remaining 1/3 (<i>n</i> = 150) were not given any distraction intervention during blood collection. Significantly lower pain scores were recorded in both intervention groups compared to the control group (<i>P</i> &lt; 0.001 and <i>P</i> &lt; 0.001).</p> Conclusion <p>Less pain was noted in the Buzzy and cartoon groups compared to the control group. This suggests that these methods are effective non-pharmacological methods that can be used to reduce pain. The results show that these methods can be safely used for venipuncture pain in children aged 9–13 years.</p>