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Objective <p>Gestational diabetes mellitus (GDM) is characterized by the onset of abnormal glucose metabolism during pregnancy. This study aims to examine the expression patterns and functional significance of miR-484 in GDM.</p> Methods <p>The levels of miR-484 in GDM patients were compared to those in normal pregnant women through RT-qPCR. Subsequently, the diagnostic and predictive capacity of miR-484 for GDM was assessed utilizing Receiver Operating Characteristic (ROC) curve. In the next phase, the effects of miR-484 on blood glucose levels and insulin resistance in GDM mice were studied by establishing a GDM mouse model. Finally, pancreatic tissues were isolated to elucidate the influence of miR-484 on inflammation processes and oxidative stress.</p> Results <p>Elevated levels of miR-484 have been observed in GDM patients, demonstrating a positive correlation with blood glucose levels. ROC curve&#xa0;analysis indicated that miR-484 possesses substantial diagnostic and predictive capabilities for GDM, with an AUC of 0.835, a&#xa0;sensitivity of 80.95%, and a&#xa0;specificity of 82.00%. Furthermore, in GDM models, the downregulation of miR-484 significantly mitigated the adverse effects associated with GDM, leading to notable reductions in blood glucose levels and insulin resistance. Following the down-regulation of miR-484, a notable reduction in the levels of IL-1β, TNF-α, IL-6, and MDA in the pancreas was also&#xa0;observed. Concurrently, there was a significant increase in the levels of SOD and CAT.</p> Conclusion <p>miR-484 might act as a biomarker for the early-stage recognition of GDM. Down-regulating this molecule is beneficial for lowering blood glucose, insulin resistance, and pancreatic inflammation or oxidative stress.</p>

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The expression trend and regulatory role of miR-484 in patients with gestational diabetes mellitus

  • 期刊论文

  • 发表日期：

    2025年8月28日

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  • Yan Hou,
  • Li Wan,
  • Lijia Long

摘要

Objective

Gestational diabetes mellitus (GDM) is characterized by the onset of abnormal glucose metabolism during pregnancy. This study aims to examine the expression patterns and functional significance of miR-484 in GDM.

Methods

The levels of miR-484 in GDM patients were compared to those in normal pregnant women through RT-qPCR. Subsequently, the diagnostic and predictive capacity of miR-484 for GDM was assessed utilizing Receiver Operating Characteristic (ROC) curve. In the next phase, the effects of miR-484 on blood glucose levels and insulin resistance in GDM mice were studied by establishing a GDM mouse model. Finally, pancreatic tissues were isolated to elucidate the influence of miR-484 on inflammation processes and oxidative stress.

Results

Elevated levels of miR-484 have been observed in GDM patients, demonstrating a positive correlation with blood glucose levels. ROC curve analysis indicated that miR-484 possesses substantial diagnostic and predictive capabilities for GDM, with an AUC of 0.835, a sensitivity of 80.95%, and a specificity of 82.00%. Furthermore, in GDM models, the downregulation of miR-484 significantly mitigated the adverse effects associated with GDM, leading to notable reductions in blood glucose levels and insulin resistance. Following the down-regulation of miR-484, a notable reduction in the levels of IL-1β, TNF-α, IL-6, and MDA in the pancreas was also observed. Concurrently, there was a significant increase in the levels of SOD and CAT.

Conclusion

miR-484 might act as a biomarker for the early-stage recognition of GDM. Down-regulating this molecule is beneficial for lowering blood glucose, insulin resistance, and pancreatic inflammation or oxidative stress.