<p>Nanomaterials as antimicrobial agents are a highly promising and auspicious way to address the challenges where traditional antimicrobial agents are ineffective. Recently, studies have revealed that, due to a large surface area, low cytotoxicity, and excellent biocompatibility, molybdenum disulfide (MoS₂) has been extensively studied for antimicrobial studies. However, engineering the nano-architectures and/or composites to enhance the antimicrobial activity is desirable to benefit their practical applications. In this context, here we demonstrate an approach based on the hybridization of MoS₂ with Fe₃O₄, where the MoS₂-Fe₃O₄ nanocomposite exhibits superior antimicrobial activity compared to MoS₂. Both were synthesized using a simple, economically viable hydrothermal method and examined for their structure, chemical bonding, surface morphology, and antimicrobial activity. The antimicrobial studies were performed using the agar diffusion method against the <i>Escherichia coli</i> (<i>E. coli</i>) and the <i>Saccharomyces cerevisiae</i> (<i>S. cerevisiae</i>). The inhibition zones measured as a result of antimicrobial activity using MoS₂ and MoS₂-Fe₃O₄ against <i>E. coli</i> were 18 and 23&#xa0;mm, while those of <i>S. cerevisiae</i> were 11 and 22&#xa0;mm, respectively. These results demonstrate that hybridizing MoS₂ (flower-like) with Fe₃O₄ (rod-like) can increase the antimicrobial properties to a large extent. This research suggests that the MoS₂-Fe₃O₄ nanocomposite holds promise as an advanced antimicrobial agent.</p>