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<p><i>Agasicles hygrophila</i> is a monophagous beetle that exclusively feeds on the host plant <i>Alternanthera philoxeroides</i>. It cannot complete its life cycle on non-target plants, so it is an effective biological control agent. However, the molecular mechanisms underlying its host specificity remain unclear due to limited transcriptomic resources. Here, we employed the Illumina HiSeq™ 2000 platform to generate transcriptomic databases for adult <i>A. hygrophila</i> under three conditions: feeding on the host plant, non-target plant <i>Beta vulgaris</i> var. <i>cicla</i> and starvation as control. We assembled 46,151 unigenes (N50 = 1312 bp). BLAST annotation against the Nr database identified <i>Tribolium castaneum</i> Herbst as the top hit. We identified 221 digestive enzyme genes (63 carbohydrases, 72 lipases, 86 trypsins) and 138 detoxification enzymes (69 P450s, 23 GSTs, 46 CCEs). KEGG analysis showed detoxification enzymes were enriched in hormone biosynthesis and metabolism. Comparative analysis of DEGs among the three treatment groups, <i>A. hygrophila</i> feeding on the host plant, exhibited a higher number of DEGs (1525 vs. 733), and higher digestive/detoxification enzyme abundances than the non-target plant. Notably, carbohydrases/lipases showed more downregulated genes, while proteases exhibited the opposite trend; P450s were the most differentially expressed detoxification enzymes. qRT-PCR validated 26 digestive and 21 detoxification enzyme DEGs with complete sequences. This study provides a comprehensive transcriptomic resource and offers mechanistic insight into the metabolic basis of monophagy in this biocontrol agent.</p> Graphical Abstract <p></p>

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Transcriptomic analysis of digestive and detoxification enzyme genes in Agasicles hygrophila (Coleoptera: Chrysomelidae) under varied dietary conditions

  • 期刊论文

  • 发表日期：

    2026年3月13日

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  • Wenjie He,
  • Shuang Zhan,
  • Cenyue Li,
  • Wenzhe Wang,
  • Yuting Wu,
  • Chun Yang,
  • Lingling Gao,
  • Ruiyan Ma,
  • Yanqiong Guo

摘要

Agasicles hygrophila is a monophagous beetle that exclusively feeds on the host plant Alternanthera philoxeroides. It cannot complete its life cycle on non-target plants, so it is an effective biological control agent. However, the molecular mechanisms underlying its host specificity remain unclear due to limited transcriptomic resources. Here, we employed the Illumina HiSeq™ 2000 platform to generate transcriptomic databases for adult A. hygrophila under three conditions: feeding on the host plant, non-target plant Beta vulgaris var. cicla and starvation as control. We assembled 46,151 unigenes (N50 = 1312 bp). BLAST annotation against the Nr database identified Tribolium castaneum Herbst as the top hit. We identified 221 digestive enzyme genes (63 carbohydrases, 72 lipases, 86 trypsins) and 138 detoxification enzymes (69 P450s, 23 GSTs, 46 CCEs). KEGG analysis showed detoxification enzymes were enriched in hormone biosynthesis and metabolism. Comparative analysis of DEGs among the three treatment groups, A. hygrophila feeding on the host plant, exhibited a higher number of DEGs (1525 vs. 733), and higher digestive/detoxification enzyme abundances than the non-target plant. Notably, carbohydrases/lipases showed more downregulated genes, while proteases exhibited the opposite trend; P450s were the most differentially expressed detoxification enzymes. qRT-PCR validated 26 digestive and 21 detoxification enzyme DEGs with complete sequences. This study provides a comprehensive transcriptomic resource and offers mechanistic insight into the metabolic basis of monophagy in this biocontrol agent.

Graphical Abstract